Kinesin-1 Prevents Capture of the Oocyte Meiotic Spindle by the Sperm Aster

Karen L P McNally, Amy S. Fabritius, Marina L. Ellefson, Jonathan R. Flynn, Jennifer A. Milan, Francis J. McNally

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Centrioles are lost during oogenesis and inherited from the sperm at fertilization. In the zygote, the centrioles recruit pericentriolar proteins from the egg to form a mature centrosome that nucleates a sperm aster. The sperm aster then captures the female pronucleus to join the maternal and paternal genomes. Because fertilization occurs before completion of female meiosis, some mechanism must prevent capture of the meiotic spindle by the sperm aster. Here we show that in wild-type . Caenorhabditis elegans zygotes, maternal pericentriolar proteins are not recruited to the sperm centrioles until after completion of meiosis. Depletion of kinesin-1 heavy chain or its binding partner resulted in premature centrosome maturation during meiosis and growth of a sperm aster that could capture the oocyte meiotic spindle. Kinesin prevents recruitment of pericentriolar proteins by coating the sperm DNA and centrioles and thus prevents triploidy by a nonmotor mechanism. Because fertilization occurs before completion of oocyte meiosis, female meiotic spindles must be protected from interference from sperm asters. McNally et al. show that in . Caenorhabditis elegans zygotes, kinesin-1 prevents sperm aster formation by blocking recruitment of maternal pericentriolar proteins to the paternal centrioles until after completion of meiosis.

Original languageEnglish (US)
Pages (from-to)788-798
Number of pages11
JournalDevelopmental Cell
Volume22
Issue number4
DOIs
StatePublished - Apr 17 2012

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Kinesin
Spindle Apparatus
Oocytes
Spermatozoa
Centrioles
Meiosis
Egg Proteins
Proteins
Zygote
Fertilization
Centrosome
Genes
Mothers
Caenorhabditis elegans
Coatings
DNA
Triploidy
Oogenesis
Genome

ASJC Scopus subject areas

  • Developmental Biology

Cite this

McNally, K. L. P., Fabritius, A. S., Ellefson, M. L., Flynn, J. R., Milan, J. A., & McNally, F. J. (2012). Kinesin-1 Prevents Capture of the Oocyte Meiotic Spindle by the Sperm Aster. Developmental Cell, 22(4), 788-798. https://doi.org/10.1016/j.devcel.2012.01.010

Kinesin-1 Prevents Capture of the Oocyte Meiotic Spindle by the Sperm Aster. / McNally, Karen L P; Fabritius, Amy S.; Ellefson, Marina L.; Flynn, Jonathan R.; Milan, Jennifer A.; McNally, Francis J.

In: Developmental Cell, Vol. 22, No. 4, 17.04.2012, p. 788-798.

Research output: Contribution to journalArticle

McNally, KLP, Fabritius, AS, Ellefson, ML, Flynn, JR, Milan, JA & McNally, FJ 2012, 'Kinesin-1 Prevents Capture of the Oocyte Meiotic Spindle by the Sperm Aster', Developmental Cell, vol. 22, no. 4, pp. 788-798. https://doi.org/10.1016/j.devcel.2012.01.010
McNally KLP, Fabritius AS, Ellefson ML, Flynn JR, Milan JA, McNally FJ. Kinesin-1 Prevents Capture of the Oocyte Meiotic Spindle by the Sperm Aster. Developmental Cell. 2012 Apr 17;22(4):788-798. https://doi.org/10.1016/j.devcel.2012.01.010
McNally, Karen L P ; Fabritius, Amy S. ; Ellefson, Marina L. ; Flynn, Jonathan R. ; Milan, Jennifer A. ; McNally, Francis J. / Kinesin-1 Prevents Capture of the Oocyte Meiotic Spindle by the Sperm Aster. In: Developmental Cell. 2012 ; Vol. 22, No. 4. pp. 788-798.
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