Kinesin-1 mediates translocation of the meiotic spindle to the oocyte cortex through KCA-1, a novel cargo adapter

Hsin Ya Yang, Paul E. Mains, Francis J. McNally

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

In animals, female meiotic spindles are attached to the egg cortex in a perpendicular orientation at anaphase to allow the selective disposal of three haploid chromosome sets into polar bodies. We have identified a complex of interacting Caenorhabditis elegans proteins that are involved in the earliest step in asymmetric positioning of anastral meiotic spindles, translocation to the cortex. This complex is composed of the kinesin-1 heavy chain orthologue, UNC-116, the kinesin light chain orthologues, KLC-1 and -2, and a novel cargo adaptor, KCA-1. Depletion of any of these subunits by RNA interference resulted in meiosis I metaphase spindles that remained stationary at a position several micrometers from the cell cortex during the time when wild-type spindles translocated to the cortex. After this prolonged stationary period, unc-116(RNAi) spindles moved to the cortex through a partially redundant mechanism that is dependent on the anaphase-promoting complex. This study thus reveals two sequential mechanisms for translocating anastral spindles to the oocyte cortex.

Original languageEnglish (US)
Pages (from-to)447-457
Number of pages11
JournalJournal of Cell Biology
Volume169
Issue number3
DOIs
StatePublished - May 9 2005

Fingerprint

Kinesin
Spindle Apparatus
RNA Interference
Oocytes
Caenorhabditis elegans Proteins
Anaphase-Promoting Complex-Cyclosome
Polar Bodies
Anaphase
Haploidy
Meiosis
Metaphase
Ovum
Chromosomes
Light

ASJC Scopus subject areas

  • Cell Biology

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Kinesin-1 mediates translocation of the meiotic spindle to the oocyte cortex through KCA-1, a novel cargo adapter. / Yang, Hsin Ya; Mains, Paul E.; McNally, Francis J.

In: Journal of Cell Biology, Vol. 169, No. 3, 09.05.2005, p. 447-457.

Research output: Contribution to journalArticle

Yang, Hsin Ya ; Mains, Paul E. ; McNally, Francis J. / Kinesin-1 mediates translocation of the meiotic spindle to the oocyte cortex through KCA-1, a novel cargo adapter. In: Journal of Cell Biology. 2005 ; Vol. 169, No. 3. pp. 447-457.
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