Kilohertz retinal FF-SS-OCT and flood imaging with hardware-based adaptive optics

Denise Valente, Kari V. Vienola, Robert J. Zawadzki, Ravi Jonnal

Research output: Contribution to journalArticlepeer-review

Abstract

A retinal imaging system was designed for full-field (FF) swept-source (SS) optical coherence tomography (OCT) with cellular resolution. The system incorporates a real-time adaptive optics (AO) subsystem and a very high-speed CMOS sensor, and is capable of acquiring volumetric images of the retina at rates up to 1 kHz. While digital aberration correction (DAC) is an attractive potential alternative to AO, it has not yet been shown to provide resolution allowing visualization of cones in the fovea, where early detection of functional deficits is most critical. Here we demonstrate that FF-SS-OCT with hardware AO permits resolution of foveal cones, imaged at eccentricities of 1° and 2°, with volume rates adequate to measure light-evoked changes in photoreceptors. With the reference arm blocked, the system can operate as a kilohertz AO flood illumination fundus camera with adjustable temporal coherence and is expected to allow measurement of light-evoked changes caused by common path interference in photoreceptor outer segments (OS). In this paper, we describe the system’s optical design, characterize its performance, and demonstrate its ability to produce images of the human photoreceptor mosaic.

Original languageEnglish (US)
Pages (from-to)5995-6011
Number of pages17
JournalBiomedical Optics Express
Volume11
Issue number10
DOIs
StatePublished - Oct 1 2020

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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