Abstract
Phosphorylation modulates many cellular processes during cell cycle progression. The yeast centrosome (called the spindle pole body, SPB) is regulated by the protein kinases Mps1 and Cdc28/Cdk1 as it nucleates microtubules to separate chromosomes during mitosis. Previously we completed an SPB phosphoproteome, identifying 297 sites on 17 of the 18 SPB components. Here we describe mutagenic analysis of phosphorylation events on Spc29 and Spc42, two SPB core components that were shown in the phosphoproteome to be heavily phosphorylated. Mutagenesis at multiple sites in Spc29 and Spc42 suggests that much of the phosphorylation on these two proteins is not essential but enhances several steps of mitosis. Of the 65 sites examined on both proteins, phosphorylation of the Mps1 sites Spc29-T18 and Spc29-T240 was shown to be critical for function. Interestingly, these two sites primarily influence distinct successive steps; Spc29-T240 is important for the interaction of Spc29 with Spc42, likely during satellite formation, and Spc29-T18 facilitates insertion of the new SPB into the nuclear envelope and promotes anaphase spindle elongation. Phosphorylation sites within Cdk1 motifs affect function to varying degrees, but mutations only have significant effects in the presence of an MPS1 mutation, supporting a theme of coregulation by these two kinases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2280-2291 |
| Number of pages | 12 |
| Journal | Molecular Biology of the Cell |
| Volume | 29 |
| Issue number | 19 |
| DOIs | |
| State | Published - Sep 15 2018 |
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ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
Cite this
Key phosphorylation events in Spc29 and Spc42 guide multiple steps of yeast centrosome duplication. / Jones, Michele Haltiner; O'Toole, Eileen T.; Fabritius, Amy S.; Muller, Eric G.; Meehl, Janet B.; Jaspersen, Sue L.; Winey, Mark.
In: Molecular Biology of the Cell, Vol. 29, No. 19, 15.09.2018, p. 2280-2291.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Key phosphorylation events in Spc29 and Spc42 guide multiple steps of yeast centrosome duplication
AU - Jones, Michele Haltiner
AU - O'Toole, Eileen T.
AU - Fabritius, Amy S.
AU - Muller, Eric G.
AU - Meehl, Janet B.
AU - Jaspersen, Sue L.
AU - Winey, Mark
PY - 2018/9/15
Y1 - 2018/9/15
N2 - Phosphorylation modulates many cellular processes during cell cycle progression. The yeast centrosome (called the spindle pole body, SPB) is regulated by the protein kinases Mps1 and Cdc28/Cdk1 as it nucleates microtubules to separate chromosomes during mitosis. Previously we completed an SPB phosphoproteome, identifying 297 sites on 17 of the 18 SPB components. Here we describe mutagenic analysis of phosphorylation events on Spc29 and Spc42, two SPB core components that were shown in the phosphoproteome to be heavily phosphorylated. Mutagenesis at multiple sites in Spc29 and Spc42 suggests that much of the phosphorylation on these two proteins is not essential but enhances several steps of mitosis. Of the 65 sites examined on both proteins, phosphorylation of the Mps1 sites Spc29-T18 and Spc29-T240 was shown to be critical for function. Interestingly, these two sites primarily influence distinct successive steps; Spc29-T240 is important for the interaction of Spc29 with Spc42, likely during satellite formation, and Spc29-T18 facilitates insertion of the new SPB into the nuclear envelope and promotes anaphase spindle elongation. Phosphorylation sites within Cdk1 motifs affect function to varying degrees, but mutations only have significant effects in the presence of an MPS1 mutation, supporting a theme of coregulation by these two kinases.
AB - Phosphorylation modulates many cellular processes during cell cycle progression. The yeast centrosome (called the spindle pole body, SPB) is regulated by the protein kinases Mps1 and Cdc28/Cdk1 as it nucleates microtubules to separate chromosomes during mitosis. Previously we completed an SPB phosphoproteome, identifying 297 sites on 17 of the 18 SPB components. Here we describe mutagenic analysis of phosphorylation events on Spc29 and Spc42, two SPB core components that were shown in the phosphoproteome to be heavily phosphorylated. Mutagenesis at multiple sites in Spc29 and Spc42 suggests that much of the phosphorylation on these two proteins is not essential but enhances several steps of mitosis. Of the 65 sites examined on both proteins, phosphorylation of the Mps1 sites Spc29-T18 and Spc29-T240 was shown to be critical for function. Interestingly, these two sites primarily influence distinct successive steps; Spc29-T240 is important for the interaction of Spc29 with Spc42, likely during satellite formation, and Spc29-T18 facilitates insertion of the new SPB into the nuclear envelope and promotes anaphase spindle elongation. Phosphorylation sites within Cdk1 motifs affect function to varying degrees, but mutations only have significant effects in the presence of an MPS1 mutation, supporting a theme of coregulation by these two kinases.
UR - http://www.scopus.com/inward/record.url?scp=85053395964&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053395964&partnerID=8YFLogxK
U2 - 10.1091/mbc.E18-05-0296
DO - 10.1091/mbc.E18-05-0296
M3 - Article
C2 - 30044722
AN - SCOPUS:85053395964
VL - 29
SP - 2280
EP - 2291
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
SN - 1059-1524
IS - 19
ER -