Keratinocyte transglutaminase promoter analysis: Identification of a functional response element

Loredana Mariniello, Qin Qin, Bart A. Jessen, Robert H. Rice

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Keratinocyte transglutaminase catalyzes isopeptide bond formation to yield the highly insoluble cross-linked envelope during terminal differentiation of epidermal cells. Transcriptional response elements were identified in the 5'- flanking DNA of the gene for this enzyme by a combination of transient transfection and electrophoretic mobility shift analyses. Since human keratinocytes transcribed ineffectively transfected transglutaminase flanking DNA, a key feature of these experiments was the use of rat bladder epithelial cells as recipients. Serial deletion experiments identified by transient transfection an important response region containing three putative AP2-like response elements approximately 0.5 kilobases from the transcription initiation site. Oligonucleotides, each containing a single one of the elements, formed specific complexes with keratinocyte nuclear proteins. Two of the response elements were found to be functional by transfection in site- specific deletion experiments. Of these one formed specific DNA-protein complexes with nuclear proteins only from cells exhibiting keratinocyte differentiation. UV cross-linking experiments estimated the protein component of the complex to be ≃85 kDa. This response element alone increased substantially the transcription of a minimal transglutaminase promoter in transient transfections. Further characterization of the putative transcription factor binding to this response element may provide insight into the regulation of keratinocyte transglutaminase.

Original languageEnglish (US)
Pages (from-to)31358-31363
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number52
DOIs
StatePublished - Dec 29 1995

Fingerprint

Transglutaminases
Response Elements
Keratinocytes
Transfection
Nuclear Proteins
DNA
Experiments
Electrophoretic mobility
Transcription Initiation Site
Transcription
Oligonucleotides
Rats
Cell Differentiation
Urinary Bladder
Proteins
Transcription Factors
Genes
Epithelial Cells
Cells
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Keratinocyte transglutaminase promoter analysis : Identification of a functional response element. / Mariniello, Loredana; Qin, Qin; Jessen, Bart A.; Rice, Robert H.

In: Journal of Biological Chemistry, Vol. 270, No. 52, 29.12.1995, p. 31358-31363.

Research output: Contribution to journalArticle

Mariniello, Loredana ; Qin, Qin ; Jessen, Bart A. ; Rice, Robert H. / Keratinocyte transglutaminase promoter analysis : Identification of a functional response element. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 52. pp. 31358-31363.
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