Katanin is responsible for the M-phase microtubulesevering activity in Xenopus eggs

Francis J. McNally, Susan Thomas

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Microtubules are dynamic structures whose proper rearrangement during the cell cycle is essential for the positioning of membranes during interphase and for chromosome segregation during mitosis. The previous discovery of a cyclin B/cdc2-activated microtubule-severing activity in M- phase Xenopus egg extracts suggested that a microtubule-severing protein might play an important role in cell cycle-dependent changes in microtubule dynamics and organization. However, the isolation of three different microtubule-severing proteins, p56, EF1α, and katanin, has only confused the issue because none of these proteins is directly activated by cyclin B/cdc2. Here we use immunodepletion with antibodies specific for a vertebrate katanin homologue to demonstrate that katanin is responsible for the majority of M- phase severing activity in Xenopus eggs. This result suggests that katanin is responsible for changes in microtubules occurring at mitosis. Immunofluorescence analysis demonstrated that katanin is concentrated at a microtubule-dependent structure at mitotic spindle poles in Xenopus A6 cells and in human fibroblasts, suggesting a specific role in microtubule disassembly at spindle poles. Surprisingly, katanin was also found in adult mouse brain, indicating that katanin may have other functions distinct from its mitotic role.

Original languageEnglish (US)
Pages (from-to)1847-1861
Number of pages15
JournalMolecular Biology of the Cell
Volume9
Issue number7
StatePublished - 1998

Fingerprint

Xenopus
Cell Division
Eggs
Microtubules
Spindle Poles
Microtubule Proteins
Cyclin B
Mitosis
Cell Cycle
Chromosome Segregation
Spindle Apparatus
Interphase
katanin
Fluorescent Antibody Technique
Ovum
Vertebrates
Fibroblasts
Membranes
Antibodies
Brain

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Katanin is responsible for the M-phase microtubulesevering activity in Xenopus eggs. / McNally, Francis J.; Thomas, Susan.

In: Molecular Biology of the Cell, Vol. 9, No. 7, 1998, p. 1847-1861.

Research output: Contribution to journalArticle

McNally, Francis J. ; Thomas, Susan. / Katanin is responsible for the M-phase microtubulesevering activity in Xenopus eggs. In: Molecular Biology of the Cell. 1998 ; Vol. 9, No. 7. pp. 1847-1861.
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