Katanin controls mitotic and meiotic spindle length

Karen McNally, Anjon Audhya, Karen Oegema, Francis J. McNally

Research output: Contribution to journalArticle

199 Scopus citations

Abstract

Accurate control of spindle length is a conserved feature of eukaryotic cell division. Lengthening of mitotic spindles contributes to chromosome segregation and cytokinesis during mitosis in animals and fungi. In contrast, spindle shortening may contribute to conservation of egg cytoplasm during female meiosis. Katanin is a microtubule-severing enzyme that is concentrated at mitotic and meiotic spindle poles in animals. We show that inhibition of katanin slows the rate of spindle shortening in nocodazole-treated mammalian fibroblasts and in untreated Caenorhabditis elegans meiotic embryos. Wildtype C. elegans meiotic spindle shortening proceeds through an early katanin-independent phase marked by increasing microtubule density and a second, katanin-dependent phase that occurs after microtubule density stops increasing. In addition, double-mutant analysis indicated that γ-tubulin-dependent nucleation and microtubule severing may provide redundant mechanisms for increasing microtubule number during the early stages of meiotic spindle assembly.

Original languageEnglish (US)
Pages (from-to)881-891
Number of pages11
JournalJournal of Cell Biology
Volume175
Issue number6
DOIs
StatePublished - Dec 18 2006

ASJC Scopus subject areas

  • Cell Biology

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    McNally, K., Audhya, A., Oegema, K., & McNally, F. J. (2006). Katanin controls mitotic and meiotic spindle length. Journal of Cell Biology, 175(6), 881-891. https://doi.org/10.1083/jcb.200608117