Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit

James J. Hartman, Jeff Mahr, Karen McNally, Katsuya Okawa, Akihiro Iwamatsu, Susan Thomas, Sarah Cheesman, John Heuser, Ronald D. Vale, Francis J. McNally

Research output: Contribution to journalArticle

245 Scopus citations

Abstract

Microtubule disassembly at centrosomes is involved in mitotic spindle function. The microtubule-severing protein katanin, a heterodimer of 60 and 80 kDa subunits, was previously purified and shown to localize to centrosomes in vive. Here we report the sequences and activities of the katanin subunits. p60 is a new member of the AAA family of ATPases, and we show that expressed 1360 has microtubule-stimulated ATPase and microtubule-severing activities in the absence of p80. 1380 is a novel protein containing WD40 repeats, which are frequently involved in protein-protein interactions. The 1380 WD40 domain does not participate in 1360 dimerization, but localizes to centrosomes in transfected mammalian cells. These results indicate katanin's activities are segregated into a subunit (p60) that possesses enzymatic activity and a subunit (p80) that targets the enzyme to the centrosome.

Original languageEnglish (US)
Pages (from-to)277-287
Number of pages11
JournalCell
Volume93
Issue number2
DOIs
StatePublished - Apr 17 1998

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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    Hartman, J. J., Mahr, J., McNally, K., Okawa, K., Iwamatsu, A., Thomas, S., Cheesman, S., Heuser, J., Vale, R. D., & McNally, F. J. (1998). Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit. Cell, 93(2), 277-287. https://doi.org/10.1016/S0092-8674(00)81578-0