Kainic acid-induced seizures stimulate increased expression of nerve growth factor mRNA in rat hippocampus

Christine Gall, Karl D Murray, Paul J. Isackson

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

The influence of kainic acid (KA)-induced limbic seizure activity on the expression of mRNA for nerve growth factor (NGF) in adult rat brain was studied using in situ hybridization and S1 nuclease protection techniques with RNA probes complementary to murine and rat NGF mRNA. Within hippocampus, intracerebroventricular injection of 0.5 μg KA caused a dramatic bilateral increase in hybridization of the 35S-labeled cRNA within stratum granulosum. This increase was first evident 1 h post-KA, appeared maximal at approximately 20-fold control levels at 2-3 h post-injection, and declined to control levels by 48 h post-injection. During the period of maximal hybridization, all but the deepest cells within stratum granulosum appeared to be autoradiographically labeled. Hybridization of the NGF cRNA probe was also increased within superficial layers of piriform and entorhinal cortex and, to much lesser extent, within scattered neurons of layers II and III of neocortex in KA-treated rats. In olfactory cortical areas, hybridization was maximally elevated 15.5-24.5 h after KA injection. In contrast to these effects, KA treatment did not consistently influence the density of hybridization, or number of neurons labeled, within the dentate gyrus hilus or the hippocampus proper (CA1-CA3). In agreement with the in situ hybridization results, S1 nuclease protection assay detected KA-induced increases in hybridization within pooled dentate gyrus/CA1 samples, but not hippocampal CA3 samples. These data support the conclusion that seizure activity stimulates a transient increase in NGF expression by select populations of forebrain neurons and indicates that experimental seizure paradigms might be further exploited for analyses of the mechanisms of NGF regulation and processing in the adult brain.

Original languageEnglish (US)
Pages (from-to)113-123
Number of pages11
JournalMolecular Brain Research
Volume9
Issue number1-2
DOIs
StatePublished - 1991

Fingerprint

Kainic Acid
Nerve Growth Factor
Hippocampus
Seizures
Messenger RNA
Complementary RNA
Injections
Dentate Gyrus
Neurons
In Situ Hybridization
Nuclease Protection Assays
RNA Probes
Entorhinal Cortex
Neocortex
Brain
Prosencephalon
Population

Keywords

  • Dentate gyrus
  • Epilepsy
  • Gene expression
  • Hippocampus
  • In situ hybridization
  • Nerve growth factor
  • Nuclease protection
  • Trophic factor

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Kainic acid-induced seizures stimulate increased expression of nerve growth factor mRNA in rat hippocampus. / Gall, Christine; Murray, Karl D; Isackson, Paul J.

In: Molecular Brain Research, Vol. 9, No. 1-2, 1991, p. 113-123.

Research output: Contribution to journalArticle

@article{20694082f8654772bb945eee34310cd9,
title = "Kainic acid-induced seizures stimulate increased expression of nerve growth factor mRNA in rat hippocampus",
abstract = "The influence of kainic acid (KA)-induced limbic seizure activity on the expression of mRNA for nerve growth factor (NGF) in adult rat brain was studied using in situ hybridization and S1 nuclease protection techniques with RNA probes complementary to murine and rat NGF mRNA. Within hippocampus, intracerebroventricular injection of 0.5 μg KA caused a dramatic bilateral increase in hybridization of the 35S-labeled cRNA within stratum granulosum. This increase was first evident 1 h post-KA, appeared maximal at approximately 20-fold control levels at 2-3 h post-injection, and declined to control levels by 48 h post-injection. During the period of maximal hybridization, all but the deepest cells within stratum granulosum appeared to be autoradiographically labeled. Hybridization of the NGF cRNA probe was also increased within superficial layers of piriform and entorhinal cortex and, to much lesser extent, within scattered neurons of layers II and III of neocortex in KA-treated rats. In olfactory cortical areas, hybridization was maximally elevated 15.5-24.5 h after KA injection. In contrast to these effects, KA treatment did not consistently influence the density of hybridization, or number of neurons labeled, within the dentate gyrus hilus or the hippocampus proper (CA1-CA3). In agreement with the in situ hybridization results, S1 nuclease protection assay detected KA-induced increases in hybridization within pooled dentate gyrus/CA1 samples, but not hippocampal CA3 samples. These data support the conclusion that seizure activity stimulates a transient increase in NGF expression by select populations of forebrain neurons and indicates that experimental seizure paradigms might be further exploited for analyses of the mechanisms of NGF regulation and processing in the adult brain.",
keywords = "Dentate gyrus, Epilepsy, Gene expression, Hippocampus, In situ hybridization, Nerve growth factor, Nuclease protection, Trophic factor",
author = "Christine Gall and Murray, {Karl D} and Isackson, {Paul J.}",
year = "1991",
doi = "10.1016/0169-328X(91)90136-L",
language = "English (US)",
volume = "9",
pages = "113--123",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Kainic acid-induced seizures stimulate increased expression of nerve growth factor mRNA in rat hippocampus

AU - Gall, Christine

AU - Murray, Karl D

AU - Isackson, Paul J.

PY - 1991

Y1 - 1991

N2 - The influence of kainic acid (KA)-induced limbic seizure activity on the expression of mRNA for nerve growth factor (NGF) in adult rat brain was studied using in situ hybridization and S1 nuclease protection techniques with RNA probes complementary to murine and rat NGF mRNA. Within hippocampus, intracerebroventricular injection of 0.5 μg KA caused a dramatic bilateral increase in hybridization of the 35S-labeled cRNA within stratum granulosum. This increase was first evident 1 h post-KA, appeared maximal at approximately 20-fold control levels at 2-3 h post-injection, and declined to control levels by 48 h post-injection. During the period of maximal hybridization, all but the deepest cells within stratum granulosum appeared to be autoradiographically labeled. Hybridization of the NGF cRNA probe was also increased within superficial layers of piriform and entorhinal cortex and, to much lesser extent, within scattered neurons of layers II and III of neocortex in KA-treated rats. In olfactory cortical areas, hybridization was maximally elevated 15.5-24.5 h after KA injection. In contrast to these effects, KA treatment did not consistently influence the density of hybridization, or number of neurons labeled, within the dentate gyrus hilus or the hippocampus proper (CA1-CA3). In agreement with the in situ hybridization results, S1 nuclease protection assay detected KA-induced increases in hybridization within pooled dentate gyrus/CA1 samples, but not hippocampal CA3 samples. These data support the conclusion that seizure activity stimulates a transient increase in NGF expression by select populations of forebrain neurons and indicates that experimental seizure paradigms might be further exploited for analyses of the mechanisms of NGF regulation and processing in the adult brain.

AB - The influence of kainic acid (KA)-induced limbic seizure activity on the expression of mRNA for nerve growth factor (NGF) in adult rat brain was studied using in situ hybridization and S1 nuclease protection techniques with RNA probes complementary to murine and rat NGF mRNA. Within hippocampus, intracerebroventricular injection of 0.5 μg KA caused a dramatic bilateral increase in hybridization of the 35S-labeled cRNA within stratum granulosum. This increase was first evident 1 h post-KA, appeared maximal at approximately 20-fold control levels at 2-3 h post-injection, and declined to control levels by 48 h post-injection. During the period of maximal hybridization, all but the deepest cells within stratum granulosum appeared to be autoradiographically labeled. Hybridization of the NGF cRNA probe was also increased within superficial layers of piriform and entorhinal cortex and, to much lesser extent, within scattered neurons of layers II and III of neocortex in KA-treated rats. In olfactory cortical areas, hybridization was maximally elevated 15.5-24.5 h after KA injection. In contrast to these effects, KA treatment did not consistently influence the density of hybridization, or number of neurons labeled, within the dentate gyrus hilus or the hippocampus proper (CA1-CA3). In agreement with the in situ hybridization results, S1 nuclease protection assay detected KA-induced increases in hybridization within pooled dentate gyrus/CA1 samples, but not hippocampal CA3 samples. These data support the conclusion that seizure activity stimulates a transient increase in NGF expression by select populations of forebrain neurons and indicates that experimental seizure paradigms might be further exploited for analyses of the mechanisms of NGF regulation and processing in the adult brain.

KW - Dentate gyrus

KW - Epilepsy

KW - Gene expression

KW - Hippocampus

KW - In situ hybridization

KW - Nerve growth factor

KW - Nuclease protection

KW - Trophic factor

UR - http://www.scopus.com/inward/record.url?scp=0025917576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025917576&partnerID=8YFLogxK

U2 - 10.1016/0169-328X(91)90136-L

DO - 10.1016/0169-328X(91)90136-L

M3 - Article

C2 - 1708074

AN - SCOPUS:0025917576

VL - 9

SP - 113

EP - 123

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -