Juvenile hormone (JH) esterase activity but not JH epoxide hydrolase activity is downregulated in larval Adoxophyes honmai following nucleopolyhedroviruses infection

Yasumasa Saito, Shizuo G. Kamita, Bruce D. Hammock, Yasuhisa Kunimi, Maki N. Inoue, Madoka Nakai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Juvenile hormones (JHs) and ecdysteroids are critical insect developmental hormones. JH esterase (JHE) and JH epoxide hydrolase (JHEH) are JH-selective enzymes that metabolize JH and thus regulate the titer of JH. Baculoviruses are known to alter host endocrine regulation. The nucleopolyhedroviruses, AdhoNPV and AdorNPV, are known to have slow and fast killing activity against Adoxophyes honmai (Lepidoptera: Tortricidae), respectively. Here we found that when penultimate (4th) instar A. honmai are inoculated with AdhoNPV or AdorNPV, the mean survival time is 9.7 and 8.2. days, respectively. The larvae molted once but did not pupate. The AdhoNPV- or AdorNPV-infected larvae did not show a dramatic increase in JHE activity as was found in mock-infected larvae, instead they showed a marked decrease in JHE activity. In contrast, both viral infections had no effect on JHEH activity. In order to further characterize the JHE activity, the JHE-coding sequence of A. honmai (ahjhe) was cloned and confirmed to encode a biologically active JHE. Quantitative real-time PCR analysis of ahjhe expression in 4th and 5th instar A. honmai revealed that AdhoNPV and AdorNPV are able to reduce ahjhe expression levels.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalJournal of Insect Physiology
Volume80
DOIs
StatePublished - Sep 1 2015

Fingerprint

Adoxophyes honmai nucleopolyhedrovirus
epoxide hydrolase
juvenile hormone esterase
Nucleopolyhedrovirus
Adoxophyes honmai
juvenile hormones
Juvenile Hormones
Down-Regulation
Infection
infection
Larva
Baculoviridae
larvae
instars
Insect Hormones
Ecdysteroids
Lepidoptera
ecdysteroids
Tortricidae
Virus Diseases

Keywords

  • Adoxophyes honmai
  • Baculovirus
  • JH epoxide hydrolase (JHEH)
  • Juvenile hormone esterase (JHE)
  • Nucleopolyhedrovirus
  • qRT-PCR

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Juvenile hormone (JH) esterase activity but not JH epoxide hydrolase activity is downregulated in larval Adoxophyes honmai following nucleopolyhedroviruses infection. / Saito, Yasumasa; Kamita, Shizuo G.; Hammock, Bruce D.; Kunimi, Yasuhisa; Inoue, Maki N.; Nakai, Madoka.

In: Journal of Insect Physiology, Vol. 80, 01.09.2015, p. 71-80.

Research output: Contribution to journalArticle

Saito, Yasumasa ; Kamita, Shizuo G. ; Hammock, Bruce D. ; Kunimi, Yasuhisa ; Inoue, Maki N. ; Nakai, Madoka. / Juvenile hormone (JH) esterase activity but not JH epoxide hydrolase activity is downregulated in larval Adoxophyes honmai following nucleopolyhedroviruses infection. In: Journal of Insect Physiology. 2015 ; Vol. 80. pp. 71-80.
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abstract = "Juvenile hormones (JHs) and ecdysteroids are critical insect developmental hormones. JH esterase (JHE) and JH epoxide hydrolase (JHEH) are JH-selective enzymes that metabolize JH and thus regulate the titer of JH. Baculoviruses are known to alter host endocrine regulation. The nucleopolyhedroviruses, AdhoNPV and AdorNPV, are known to have slow and fast killing activity against Adoxophyes honmai (Lepidoptera: Tortricidae), respectively. Here we found that when penultimate (4th) instar A. honmai are inoculated with AdhoNPV or AdorNPV, the mean survival time is 9.7 and 8.2. days, respectively. The larvae molted once but did not pupate. The AdhoNPV- or AdorNPV-infected larvae did not show a dramatic increase in JHE activity as was found in mock-infected larvae, instead they showed a marked decrease in JHE activity. In contrast, both viral infections had no effect on JHEH activity. In order to further characterize the JHE activity, the JHE-coding sequence of A. honmai (ahjhe) was cloned and confirmed to encode a biologically active JHE. Quantitative real-time PCR analysis of ahjhe expression in 4th and 5th instar A. honmai revealed that AdhoNPV and AdorNPV are able to reduce ahjhe expression levels.",
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AU - Saito, Yasumasa

AU - Kamita, Shizuo G.

AU - Hammock, Bruce D.

AU - Kunimi, Yasuhisa

AU - Inoue, Maki N.

AU - Nakai, Madoka

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