Jug r 4, a legumin group food allergen from walnut (Juglans regia Cv. Chandler)

Mikhael Wallowitz, W. Rich Peterson, Sandie Uratsu, Sarah S. Comstock, Abhaya M. Dandekar, Suzanne S Teuber

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Allergy to walnut is the most frequently reported tree nut allergy in the United States. Walnut 2S albumin, a vicilin-like protein, and a lipid transfer protein allergen have previously been described. Our objective was to clone and express a cDNA encoding a legumin group protein, assess IgE-binding with sera from walnut allergic patients, and investigate cross-reactivity with selected nuts. Primers were used to obtain the cDNA by 5′ and 3′ rapid amplification of cDNA ends from walnut mRNA. The cDNA was subcloned into the pMAL-c2X vector and the recombinant fusion protein, named rJug r 4, was expressed in Escherichia coli. The obtained cDNA encoded a precursor protein with a predicted molecular weight of 58.1 kD, which showed significant sequence homology to hazelnut and cashew legumin allergens. Serum IgE from 21 of 37 (57%) patients bound the rJug r 4 fusion protein. In vitro cross-reactivity was demonstrated with hazelnut, cashew, and peanut protein extracts.

Original languageEnglish (US)
Pages (from-to)8369-8375
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number21
DOIs
StatePublished - Oct 18 2006

Fingerprint

Juglans
legumin
Juglans regia
food groups
walnuts
allergens
Allergens
Complementary DNA
Food
cashew nuts
Anacardium
Corylus
hazelnuts
Allergies
cross reaction
nuts
hypersensitivity
proteins
recombinant fusion proteins
Reactivity (nuclear)

Keywords

  • cDNA
  • Food allergy
  • Jug r 4
  • Legumin group protein
  • Peanut allergy
  • Tree nut allergy
  • Walnut

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Jug r 4, a legumin group food allergen from walnut (Juglans regia Cv. Chandler). / Wallowitz, Mikhael; Peterson, W. Rich; Uratsu, Sandie; Comstock, Sarah S.; Dandekar, Abhaya M.; Teuber, Suzanne S.

In: Journal of Agricultural and Food Chemistry, Vol. 54, No. 21, 18.10.2006, p. 8369-8375.

Research output: Contribution to journalArticle

Wallowitz, Mikhael ; Peterson, W. Rich ; Uratsu, Sandie ; Comstock, Sarah S. ; Dandekar, Abhaya M. ; Teuber, Suzanne S. / Jug r 4, a legumin group food allergen from walnut (Juglans regia Cv. Chandler). In: Journal of Agricultural and Food Chemistry. 2006 ; Vol. 54, No. 21. pp. 8369-8375.
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