Abstract
Allergy to walnut is the most frequently reported tree nut allergy in the United States. Walnut 2S albumin, a vicilin-like protein, and a lipid transfer protein allergen have previously been described. Our objective was to clone and express a cDNA encoding a legumin group protein, assess IgE-binding with sera from walnut allergic patients, and investigate cross-reactivity with selected nuts. Primers were used to obtain the cDNA by 5′ and 3′ rapid amplification of cDNA ends from walnut mRNA. The cDNA was subcloned into the pMAL-c2X vector and the recombinant fusion protein, named rJug r 4, was expressed in Escherichia coli. The obtained cDNA encoded a precursor protein with a predicted molecular weight of 58.1 kD, which showed significant sequence homology to hazelnut and cashew legumin allergens. Serum IgE from 21 of 37 (57%) patients bound the rJug r 4 fusion protein. In vitro cross-reactivity was demonstrated with hazelnut, cashew, and peanut protein extracts.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8369-8375 |
| Number of pages | 7 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 54 |
| Issue number | 21 |
| DOIs | |
| State | Published - Oct 18 2006 |
Fingerprint
Keywords
- cDNA
- Food allergy
- Jug r 4
- Legumin group protein
- Peanut allergy
- Tree nut allergy
- Walnut
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Chemistry (miscellaneous)
Cite this
Jug r 4, a legumin group food allergen from walnut (Juglans regia Cv. Chandler). / Wallowitz, Mikhael; Peterson, W. Rich; Uratsu, Sandie; Comstock, Sarah S.; Dandekar, Abhaya M.; Teuber, Suzanne S.
In: Journal of Agricultural and Food Chemistry, Vol. 54, No. 21, 18.10.2006, p. 8369-8375.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Jug r 4, a legumin group food allergen from walnut (Juglans regia Cv. Chandler)
AU - Wallowitz, Mikhael
AU - Peterson, W. Rich
AU - Uratsu, Sandie
AU - Comstock, Sarah S.
AU - Dandekar, Abhaya M.
AU - Teuber, Suzanne S
PY - 2006/10/18
Y1 - 2006/10/18
N2 - Allergy to walnut is the most frequently reported tree nut allergy in the United States. Walnut 2S albumin, a vicilin-like protein, and a lipid transfer protein allergen have previously been described. Our objective was to clone and express a cDNA encoding a legumin group protein, assess IgE-binding with sera from walnut allergic patients, and investigate cross-reactivity with selected nuts. Primers were used to obtain the cDNA by 5′ and 3′ rapid amplification of cDNA ends from walnut mRNA. The cDNA was subcloned into the pMAL-c2X vector and the recombinant fusion protein, named rJug r 4, was expressed in Escherichia coli. The obtained cDNA encoded a precursor protein with a predicted molecular weight of 58.1 kD, which showed significant sequence homology to hazelnut and cashew legumin allergens. Serum IgE from 21 of 37 (57%) patients bound the rJug r 4 fusion protein. In vitro cross-reactivity was demonstrated with hazelnut, cashew, and peanut protein extracts.
AB - Allergy to walnut is the most frequently reported tree nut allergy in the United States. Walnut 2S albumin, a vicilin-like protein, and a lipid transfer protein allergen have previously been described. Our objective was to clone and express a cDNA encoding a legumin group protein, assess IgE-binding with sera from walnut allergic patients, and investigate cross-reactivity with selected nuts. Primers were used to obtain the cDNA by 5′ and 3′ rapid amplification of cDNA ends from walnut mRNA. The cDNA was subcloned into the pMAL-c2X vector and the recombinant fusion protein, named rJug r 4, was expressed in Escherichia coli. The obtained cDNA encoded a precursor protein with a predicted molecular weight of 58.1 kD, which showed significant sequence homology to hazelnut and cashew legumin allergens. Serum IgE from 21 of 37 (57%) patients bound the rJug r 4 fusion protein. In vitro cross-reactivity was demonstrated with hazelnut, cashew, and peanut protein extracts.
KW - cDNA
KW - Food allergy
KW - Jug r 4
KW - Legumin group protein
KW - Peanut allergy
KW - Tree nut allergy
KW - Walnut
UR - http://www.scopus.com/inward/record.url?scp=33751209784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751209784&partnerID=8YFLogxK
U2 - 10.1021/jf061329s
DO - 10.1021/jf061329s
M3 - Article
C2 - 17032053
AN - SCOPUS:33751209784
VL - 54
SP - 8369
EP - 8375
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 21
ER -