Jug r 4, a legumin group food allergen from walnut (Juglans regia Cv. Chandler)

Mikhael Wallowitz, W. Rich Peterson, Sandie Uratsu, Sarah S. Comstock, Abhaya M. Dandekar, Suzanne S Teuber

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


Allergy to walnut is the most frequently reported tree nut allergy in the United States. Walnut 2S albumin, a vicilin-like protein, and a lipid transfer protein allergen have previously been described. Our objective was to clone and express a cDNA encoding a legumin group protein, assess IgE-binding with sera from walnut allergic patients, and investigate cross-reactivity with selected nuts. Primers were used to obtain the cDNA by 5′ and 3′ rapid amplification of cDNA ends from walnut mRNA. The cDNA was subcloned into the pMAL-c2X vector and the recombinant fusion protein, named rJug r 4, was expressed in Escherichia coli. The obtained cDNA encoded a precursor protein with a predicted molecular weight of 58.1 kD, which showed significant sequence homology to hazelnut and cashew legumin allergens. Serum IgE from 21 of 37 (57%) patients bound the rJug r 4 fusion protein. In vitro cross-reactivity was demonstrated with hazelnut, cashew, and peanut protein extracts.

Original languageEnglish (US)
Pages (from-to)8369-8375
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Issue number21
StatePublished - Oct 18 2006


  • cDNA
  • Food allergy
  • Jug r 4
  • Legumin group protein
  • Peanut allergy
  • Tree nut allergy
  • Walnut

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)


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