Jagged 1 is necessary for normal mouse lens formation

Tien T. Le, Kevin W. Conley, Nadean L Brown

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In mammals, two spatially and temporally distinct waves of fiber cell differentiation are crucial steps for normal lens development. In between these phases, an anterior growth zone forms in which progenitor cells migrate circumferentially, terminally exit the cell cycle and initiate differentiation at the lens equator. Much remains unknown about the molecular pathways orchestrating these processes. Previously, the Notch signal transduction pathway was shown to be critical for anterior lens progenitor cell growth and differentiation. However, the ligand or ligand(s) that direct these events are unknown. Using conditional gene targeting, we show that Jagged1 is required for lens fiber cell genesis, particularly that of secondary fiber cells. In the absence of Jagged1, the anterior growth and equatorial transition zones fail to develop fully, with only a handful of differentiated fiber cells present at birth. Adult Jagged1 conditional mutants completely lack lenses, along with severe anterior chamber deformities. Our data support the hypothesis that Jagged1-Notch signaling conveys a lateral inductive signal, which is indispensable for lens progenitor cell proliferation and differentiation.

Original languageEnglish (US)
Pages (from-to)118-126
Number of pages9
JournalDevelopmental Biology
Volume328
Issue number1
DOIs
StatePublished - Apr 1 2009
Externally publishedYes

Fingerprint

Lenses
Cell Differentiation
Stem Cells
Growth
Ligands
Gene Targeting
Anterior Chamber
Jagged-1 Protein
Mammals
Signal Transduction
Cell Cycle
Cell Proliferation
Parturition

Keywords

  • Aphakia
  • Jagged1
  • Lens development
  • Lens fiber differentiation
  • Notch signaling
  • Proliferation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Jagged 1 is necessary for normal mouse lens formation. / Le, Tien T.; Conley, Kevin W.; Brown, Nadean L.

In: Developmental Biology, Vol. 328, No. 1, 01.04.2009, p. 118-126.

Research output: Contribution to journalArticle

Le, Tien T. ; Conley, Kevin W. ; Brown, Nadean L. / Jagged 1 is necessary for normal mouse lens formation. In: Developmental Biology. 2009 ; Vol. 328, No. 1. pp. 118-126.
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