'It's hollow'

The function of pores within myoglobin

Ayana Tomita, Ulrike Kreutzer, Shin Ichi Adachi, Shin Ya Koshihara, Thomas Jue

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Despite a century of research, the cellular function of myoglobin (Mb), the mechanism regulating oxygen (O2) transport in the cell and the structure-function relationship of Mb remain incompletely understood. In particular, the presence and function of pores within Mb have attracted much recent attention. These pores can bind to Xe as well as to other ligands. Indeed, recent cryogenic X-ray crystallographic studies using novel techniques have captured snapshots of carbon monoxide (CO) migrating through these pores. The observed movement of the CO molecule from the heme iron site to the internal cavities and the associated structural changes of the amino acid residues around the cavities confirm the integral role of the pores in forming a ligand migration pathway from the protein surface to the heme. These observations resolve a long-standing controversy - but how these pores affect the physiological function of Mb poses a striking question at the frontier of biology.

Original languageEnglish (US)
Pages (from-to)2748-2754
Number of pages7
JournalJournal of Experimental Biology
Volume213
Issue number16
DOIs
StatePublished - Aug 2010

Fingerprint

myoglobin
Myoglobin
carbon monoxide
ligand
cavity
Carbon Monoxide
Heme
structural change
amino acid
Ligands
heme iron
iron
oxygen
protein
heme
structure-activity relationships
Membrane Proteins
X-radiation
Iron
X-Rays

Keywords

  • Crystallography
  • Laser
  • Ligand migration
  • NMR
  • Oxygen
  • Protein
  • Respiration
  • Seal

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science
  • Medicine(all)

Cite this

Tomita, A., Kreutzer, U., Adachi, S. I., Koshihara, S. Y., & Jue, T. (2010). 'It's hollow': The function of pores within myoglobin. Journal of Experimental Biology, 213(16), 2748-2754. https://doi.org/10.1242/jeb.042994

'It's hollow' : The function of pores within myoglobin. / Tomita, Ayana; Kreutzer, Ulrike; Adachi, Shin Ichi; Koshihara, Shin Ya; Jue, Thomas.

In: Journal of Experimental Biology, Vol. 213, No. 16, 08.2010, p. 2748-2754.

Research output: Contribution to journalArticle

Tomita, A, Kreutzer, U, Adachi, SI, Koshihara, SY & Jue, T 2010, ''It's hollow': The function of pores within myoglobin', Journal of Experimental Biology, vol. 213, no. 16, pp. 2748-2754. https://doi.org/10.1242/jeb.042994
Tomita, Ayana ; Kreutzer, Ulrike ; Adachi, Shin Ichi ; Koshihara, Shin Ya ; Jue, Thomas. / 'It's hollow' : The function of pores within myoglobin. In: Journal of Experimental Biology. 2010 ; Vol. 213, No. 16. pp. 2748-2754.
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