Isotope-labeled immunoassays without radiation waste

Guomin Shan, Wei Huang, Shirley J. Gee, Bruce A. Buchholz, John S. Vogel, Bruce D. Hammock

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The practice of immunoassay has experienced a widespread transition from radioisotopic labeling to nonisotopic labeling over the last two decades. Radioisotope labels have drawbacks that hamper their applications: (i) perceived radiation hazards of reagents, (ii) regulatory requirements and disposal problems of working with radioactive materials, and (iii) short shelf-life of the labeled reagents. The advantage of isotopic labeling is the incorporation into analytes without altering structure or reactivity, as is often the case with ELISA or fluorescent detection systems. We developed a format for isotope label immunoassay with the long-life isotope 14C as the label and accelerator mass spectrometer (AMS) as the detection system. AMS quantifies attomole levels of several isotopes, including 14C. With this exquisite sensitivity, the sensitivity of an immunoassay is limited by the K(d) of the antibody and not the detection system. The detection limit of the assays for atrazine and 2,3,7,8-tetrachlorodibenzo-p-dioxin was 2.0 x 10- 10 M and 2.0 x 10-11 M, respectively, approximately an order of magnitude below the standard enzyme immunoassay. Notably, < 1 dpm (0.45 pCi)of 14C-labeled compound was used in each assay, which is well below the limit of disposal (50 nCi per g) as nonradioactive waste. Thus, endogenous reporter ligands quantified by AMS provide the advantages of an RIA without the associated problems of radioactive waste.

Original languageEnglish (US)
Pages (from-to)2445-2449
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number6
DOIs
StatePublished - Mar 14 2000

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Immunoassay
Isotopes
Radiation
Radioactive Waste
Atrazine
Immunoenzyme Techniques
Radioisotopes
Limit of Detection
Enzyme-Linked Immunosorbent Assay
Ligands
Antibodies

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Isotope-labeled immunoassays without radiation waste. / Shan, Guomin; Huang, Wei; Gee, Shirley J.; Buchholz, Bruce A.; Vogel, John S.; Hammock, Bruce D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 6, 14.03.2000, p. 2445-2449.

Research output: Contribution to journalArticle

Shan, Guomin ; Huang, Wei ; Gee, Shirley J. ; Buchholz, Bruce A. ; Vogel, John S. ; Hammock, Bruce D. / Isotope-labeled immunoassays without radiation waste. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 6. pp. 2445-2449.
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