Isomer-specific LC/MS and LC/MS/MS profiling of the mouse serum N-glycome revealing a number of novel sialylated N-glycans

Serenus Hua, Ha Neul Jeong, Lauren M. Dimapasoc, Inae Kang, Chanyoung Han, Jong Soon Choi, Carlito B Lebrilla, Hyun Joo An

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Mice are the premier mammalian models for studies of human physiology and disease, bearing extensive biological similarity to humans with far fewer ethical, economic, or logistic complications. To facilitate glycomic studies based on the mouse model, we comprehensively profiled the mouse serum N-glycome using isomer-specific nano-LC/MS and -LC/MS/MS. N-Glycans were identified by accurate mass MS and structurally elucidated by MS/MS. Porous graphitized carbon nano-LC was able to separate out nearly 300 N-linked glycan compounds (including isomers) from just over 100 distinct N-linked glycan compositions. Additional MS/MS structural analysis was performed on a number of novel N-glycans, revealing the structural characteristics of modifications such as dehydration, O-acetylation, and lactylation. Experimental findings were combined with known glycobiology to generate a theoretical library of all biologically possible mouse serum N-glycan compositions. The library may be used for automated identification of complex mixtures of mouse N-glycans, with possible applications to a wide range of mouse-related research endeavors, including pharmaceutical drug development and biomarker discovery.

Original languageEnglish (US)
Pages (from-to)4636-4643
Number of pages8
JournalAnalytical Chemistry
Volume85
Issue number9
DOIs
StatePublished - May 7 2013

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Isomers
Polysaccharides
Bearings (structural)
Acetylation
Physiology
Biomarkers
Complex Mixtures
Chemical analysis
Dehydration
Structural analysis
Pharmaceutical Preparations
Logistics
Carbon
Economics
Glycomics

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Hua, S., Jeong, H. N., Dimapasoc, L. M., Kang, I., Han, C., Choi, J. S., ... An, H. J. (2013). Isomer-specific LC/MS and LC/MS/MS profiling of the mouse serum N-glycome revealing a number of novel sialylated N-glycans. Analytical Chemistry, 85(9), 4636-4643. https://doi.org/10.1021/ac400195h

Isomer-specific LC/MS and LC/MS/MS profiling of the mouse serum N-glycome revealing a number of novel sialylated N-glycans. / Hua, Serenus; Jeong, Ha Neul; Dimapasoc, Lauren M.; Kang, Inae; Han, Chanyoung; Choi, Jong Soon; Lebrilla, Carlito B; An, Hyun Joo.

In: Analytical Chemistry, Vol. 85, No. 9, 07.05.2013, p. 4636-4643.

Research output: Contribution to journalArticle

Hua, S, Jeong, HN, Dimapasoc, LM, Kang, I, Han, C, Choi, JS, Lebrilla, CB & An, HJ 2013, 'Isomer-specific LC/MS and LC/MS/MS profiling of the mouse serum N-glycome revealing a number of novel sialylated N-glycans', Analytical Chemistry, vol. 85, no. 9, pp. 4636-4643. https://doi.org/10.1021/ac400195h
Hua, Serenus ; Jeong, Ha Neul ; Dimapasoc, Lauren M. ; Kang, Inae ; Han, Chanyoung ; Choi, Jong Soon ; Lebrilla, Carlito B ; An, Hyun Joo. / Isomer-specific LC/MS and LC/MS/MS profiling of the mouse serum N-glycome revealing a number of novel sialylated N-glycans. In: Analytical Chemistry. 2013 ; Vol. 85, No. 9. pp. 4636-4643.
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