Isolation of Theileria parva lawrencei-infected lymphoid cell lines from free-ranging African buffaloes (Syncerus caffer).

D. A. Stagg, Patricia A Conrad, P. B. Rossiter

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Lymphoid cells collected from the peripheral blood of 45 free-ranging buffaloes sampled in the Masai Mara Game Reserve in Kenya were cultured in vitro in an attempt to establish cell lines of intralymphocytic theilerial schizonts. Theileria parva lawrencei-infected lymphoblastoid cell lines were established with samples taken from 12 buffaloes, and 11 of these were maintained continuously in vitro. Sixteen of the buffalo samples were contaminated with either trypanosomes or viruses. The successful in vitro isolation of Theileria species from 27 per cent of the buffaloes sampled demonstrates the applicability of this technique for field isolation of T parva lawrencei.

Original languageEnglish (US)
Pages (from-to)124-126
Number of pages3
JournalResearch in Veterinary Science
Volume43
Issue number1
StatePublished - Jul 1987
Externally publishedYes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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