Isolation of the origin of replication of the IncW-group plasmid pSa

Robert C. Tait, Timothy J. Close, Raymond L. Rodriguez, Clarence I. Kado

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The origin of replication of the IncW plasmid pSa has been cloned and the function of this origin in Escherichia coli examined. A 1.9-kb region of DNA is required for efficient autonomous replication, and a 0.47-kb fragment within this region can initiate replication only in the presence of an autonomously replicating derivative of pSa. An Mr 35 000 protein (repA) is encoded adjacent to the origin and is required for efficient initiation of replication. The derivatives examined provide information suggesting a direct role of partition factors in plasmid replication and incompatibility.

Original languageEnglish (US)
Pages (from-to)39-49
Number of pages11
JournalGene
Volume20
Issue number1
DOIs
StatePublished - 1982

Keywords

  • Gram-negative vectors
  • initiation of replication
  • plasmid incompatibility
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

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    Tait, R. C., Close, T. J., Rodriguez, R. L., & Kado, C. I. (1982). Isolation of the origin of replication of the IncW-group plasmid pSa. Gene, 20(1), 39-49. https://doi.org/10.1016/0378-1119(82)90085-3