Isolation of the dorsal locus of Drosophila

Ruth Steward, Francis J. McNally, Paul Schedl

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The establishment of embryonic polarity is a crucial step in pattern formation and morphogenesis. In the fruitfly Drosophila melanogaster, embryonic polarity depends primarily on genes expressed in the female during oogenesis. Mutations in these 'maternal effect' genes can lead to major disruptions in normal pattern formation. Two classes of maternal genes essential for the establishment of polarity in the embryo have been identified. Lesions in one class, the 'bicaudal' genes, disrupt the anterior-posterior axis1; lesions in the other class disrupt dorsal-ventral polarity, and in the most extreme cases embryos fail to form any ventral or lateral structures. Genetic studies suggest that the anterior-posterior and dorsal-ventral axes may be independent as the defects observed in mutants from each class seem to be restricted to one axis only2. The dorsal (dl) locus2 is one of the maternal effect genes involved in the establishment of dorsal-ventral polarity. Homozygous dl females produce embryos exhibiting the mutant phenotype - complete lack of dorsal-ventral polarity in the strongest alleles - irrespective of the genotype of the father. Although dl is a maternal effect locus and must be expressed during oogenesis, the gene product, or a substance depending on the normal function of the dl gene, seems to be active early in embryogenesis, as the dl phenotype can be partially rescued by injection of cytoplasm from wild-type cleavage-stage embryos3,4. Here we report the molecular cloning of the dorsal locus and a study of its expression.

Original languageEnglish (US)
Pages (from-to)262-265
Number of pages4
JournalNature
Volume311
Issue number5983
DOIs
StatePublished - 1984
Externally publishedYes

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Drosophila
Genes
Oogenesis
Embryonic Structures
Phenotype
Essential Genes
Molecular Cloning
Drosophila melanogaster
Morphogenesis
Fathers
Embryonic Development
Cytoplasm
Alleles
Genotype
Mothers
Mutation
Injections
Maternal Inheritance

ASJC Scopus subject areas

  • General

Cite this

Steward, R., McNally, F. J., & Schedl, P. (1984). Isolation of the dorsal locus of Drosophila. Nature, 311(5983), 262-265. https://doi.org/10.1038/311262a0

Isolation of the dorsal locus of Drosophila. / Steward, Ruth; McNally, Francis J.; Schedl, Paul.

In: Nature, Vol. 311, No. 5983, 1984, p. 262-265.

Research output: Contribution to journalArticle

Steward, R, McNally, FJ & Schedl, P 1984, 'Isolation of the dorsal locus of Drosophila', Nature, vol. 311, no. 5983, pp. 262-265. https://doi.org/10.1038/311262a0
Steward R, McNally FJ, Schedl P. Isolation of the dorsal locus of Drosophila. Nature. 1984;311(5983):262-265. https://doi.org/10.1038/311262a0
Steward, Ruth ; McNally, Francis J. ; Schedl, Paul. / Isolation of the dorsal locus of Drosophila. In: Nature. 1984 ; Vol. 311, No. 5983. pp. 262-265.
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