Isolation of simian virus 40 from rhesus monkeys (Macaca mulatta) with spontaneous progressive multifocal leukoencephalopathy.

C. A. Holmberg, D. H. Gribble, K. K. Takemoto, P. M. Howley, C. Espana, Bennie Osburn

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Isolates of virus from the brain tissue of two naturally occurring cases of progressive multifocal leukoencephalopathy in rhesus monkeys (Macaca mulatta) have been characterized. Both isolates were demonstrated to be simian virus 40 (SV40) by serological tests and analysis of cleavage fragments of viral deoxyribonucleic acid produced by restriction endonuclease from Haemophilus influenzae. SV40 virions and the nonvirion T antigen were demonstrated in the brain lesions of one monkey by the fluorescent antibody staining technique. SV40 was not demonstrated in the brain of normal rhesus monkeys from the same colony with use of the same methods of viral isolation or demonstration of antigen.

Original languageEnglish (US)
Pages (from-to)593-596
Number of pages4
JournalThe Journal of infectious diseases
Volume136
Issue number4
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

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Progressive Multifocal Leukoencephalopathy
Simian virus 40
Macaca mulatta
Brain
Viral Tumor Antigens
DNA Restriction Enzymes
Haemophilus influenzae
Serologic Tests
Virion
Haplorhini
Fluorescent Antibody Technique
Staining and Labeling
Viruses
Antigens
DNA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Isolation of simian virus 40 from rhesus monkeys (Macaca mulatta) with spontaneous progressive multifocal leukoencephalopathy. / Holmberg, C. A.; Gribble, D. H.; Takemoto, K. K.; Howley, P. M.; Espana, C.; Osburn, Bennie.

In: The Journal of infectious diseases, Vol. 136, No. 4, 01.01.1977, p. 593-596.

Research output: Contribution to journalArticle

Holmberg, C. A. ; Gribble, D. H. ; Takemoto, K. K. ; Howley, P. M. ; Espana, C. ; Osburn, Bennie. / Isolation of simian virus 40 from rhesus monkeys (Macaca mulatta) with spontaneous progressive multifocal leukoencephalopathy. In: The Journal of infectious diseases. 1977 ; Vol. 136, No. 4. pp. 593-596.
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