Isolation of Salmonella organisms from the mesenteric lymph nodes of horses at necropsy

John K. House, Bradford Smith, Terry R. Wildman, Mark J. Carrigan, Darin Y. Kamiya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives - To determine the prevalence of Salmonella infections in horses at necropsy. Design - Cross-sectional prevalence survey. Animals - 102 horses. Procedure - Mesenteric lymph nodes were collected from horses that were necropsied. Horses had died or were euthanatized because of severe disease or at the request of the owner. Twenty-eight of the horses were racehorses euthanatized following acute catastrophic injuries on the racetrack. Mesenteric lymph nodes were submitted for Salmonella culture via direct plating of tissue specimens on MacConkey agar and by use of 4 enrichment culture techniques that used tetrathionate and selenite enrichment broth and brilliant green and Salmonella-Shigella selective plating media. Results - Salmonella typhimurium was isolated from the mesenteric lymph nodes of 2 foals (2/102, 1.96% of the horses). Salmonella organisms were not isolated from the mesenteric lymph nodes of adult horses. Conclusions and Clinical Relevance - Prevalence of Salmonella infections in horses of our study (1.96%) suggests that the results of cross-sectional surveys, using bacteriologic culture to determine prevalence of Salmonella infection, should be interpreted with caution. Prevalence of Salmonella infections determined in a single facility may not reflect the prevalence of Salmonella-infected horses in the general population; furthermore, obtaining a Salmonella isolate from a horse does not establish that the horse is a chronic Salmonella carrier. (J Am Vet Med Assoc 1999;215: 507-510).

Original languageEnglish (US)
Pages (from-to)507-510
Number of pages4
JournalJournal of the American Veterinary Medical Association
Volume215
Issue number4
StatePublished - Aug 15 1999

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Salmonella
Horses
lymph nodes
necropsy
Lymph Nodes
horses
organisms
Salmonella Infections
salmonellosis
Cross-Sectional Studies
Selenious Acid
Culture Techniques
selenites
enrichment culture
Shigella
racehorses
selective media
Salmonella typhimurium
Salmonella Typhimurium
foals

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Isolation of Salmonella organisms from the mesenteric lymph nodes of horses at necropsy. / House, John K.; Smith, Bradford; Wildman, Terry R.; Carrigan, Mark J.; Kamiya, Darin Y.

In: Journal of the American Veterinary Medical Association, Vol. 215, No. 4, 15.08.1999, p. 507-510.

Research output: Contribution to journalArticle

House, John K. ; Smith, Bradford ; Wildman, Terry R. ; Carrigan, Mark J. ; Kamiya, Darin Y. / Isolation of Salmonella organisms from the mesenteric lymph nodes of horses at necropsy. In: Journal of the American Veterinary Medical Association. 1999 ; Vol. 215, No. 4. pp. 507-510.
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abstract = "Objectives - To determine the prevalence of Salmonella infections in horses at necropsy. Design - Cross-sectional prevalence survey. Animals - 102 horses. Procedure - Mesenteric lymph nodes were collected from horses that were necropsied. Horses had died or were euthanatized because of severe disease or at the request of the owner. Twenty-eight of the horses were racehorses euthanatized following acute catastrophic injuries on the racetrack. Mesenteric lymph nodes were submitted for Salmonella culture via direct plating of tissue specimens on MacConkey agar and by use of 4 enrichment culture techniques that used tetrathionate and selenite enrichment broth and brilliant green and Salmonella-Shigella selective plating media. Results - Salmonella typhimurium was isolated from the mesenteric lymph nodes of 2 foals (2/102, 1.96{\%} of the horses). Salmonella organisms were not isolated from the mesenteric lymph nodes of adult horses. Conclusions and Clinical Relevance - Prevalence of Salmonella infections in horses of our study (1.96{\%}) suggests that the results of cross-sectional surveys, using bacteriologic culture to determine prevalence of Salmonella infection, should be interpreted with caution. Prevalence of Salmonella infections determined in a single facility may not reflect the prevalence of Salmonella-infected horses in the general population; furthermore, obtaining a Salmonella isolate from a horse does not establish that the horse is a chronic Salmonella carrier. (J Am Vet Med Assoc 1999;215: 507-510).",
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