Isolation of P2 protein-reactive T-cell lines from human blood

J. Burns, L. J. Krasner, A. Rostami, David E Pleasure

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Human T-cell lines reactive with the peripheral nerve myelin protein, P2 protein, were isolated from the peripheral blood of 4 normal persons and 1 patient with Guillain-Barre syndrome. These predominantly helper phenotype T-cell lines were isolated and maintained in vitro by antigen stimulation followed by culture with interleukin 2. Myelin basic protein-reactive T cells were also isolated in parallel from the same subjects as antigen specificity controls. T cells recognizing myelin basic protein did not respond to P2 protein, nor did P2-reactive cells respond to myelin basic protein. These findings suggest that a potential for autoimmune reactivity with peripheral nervous system myelin antigens may exist for both normal persons and some patients with neurological disease.

Original languageEnglish (US)
Pages (from-to)391-393
Number of pages3
JournalAnnals of Neurology
Volume19
Issue number4
StatePublished - 1986
Externally publishedYes

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Myelin Basic Protein
T-Lymphocytes
Antigens
Cell Line
Myelin P2 Protein
Proteins
Guillain-Barre Syndrome
Peripheral Nervous System
Myelin Sheath
Helper-Inducer T-Lymphocytes
Peripheral Nerves
Interleukin-2
Phenotype

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Burns, J., Krasner, L. J., Rostami, A., & Pleasure, D. E. (1986). Isolation of P2 protein-reactive T-cell lines from human blood. Annals of Neurology, 19(4), 391-393.

Isolation of P2 protein-reactive T-cell lines from human blood. / Burns, J.; Krasner, L. J.; Rostami, A.; Pleasure, David E.

In: Annals of Neurology, Vol. 19, No. 4, 1986, p. 391-393.

Research output: Contribution to journalArticle

Burns, J, Krasner, LJ, Rostami, A & Pleasure, DE 1986, 'Isolation of P2 protein-reactive T-cell lines from human blood', Annals of Neurology, vol. 19, no. 4, pp. 391-393.
Burns, J. ; Krasner, L. J. ; Rostami, A. ; Pleasure, David E. / Isolation of P2 protein-reactive T-cell lines from human blood. In: Annals of Neurology. 1986 ; Vol. 19, No. 4. pp. 391-393.
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