Isolation of P22 specialized transducing phage following F' episome fusion

R. Kaye, J. Barravecchio, J. Roth

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


A P22 specialized transducing phage has been constructed which carries the structural gene for aspartate transcarbamylase (ATCase). This gene (pyrB) was first brought close to the P22 attachment site by fusing an F' pyrB+ episome to an F'prolac episome which carries a P22 prophage attachment site. A prophage was added to these fused F' episomes and the lysogen was UV induced. The specialized transducing phage was isolated from the resulting lysate. The phage also carries argI, the structure gene for ornithine transcarbamylase.

Original languageEnglish (US)
Pages (from-to)655-667
Number of pages13
Issue number4
StatePublished - 1974

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)


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