Abstract
Implantation of demineralized diaphyseal bone matrix in subcutaneous sites induces a sequence of events resulting in the local differentiation of endochondral bone. Demineralized bovine bone matrix was dissociatively extracted in 4.0 M guanidine hydrochloride and the bone-inductive proteins were purified greater than 12,000-fold. The purification steps include affinity chromatography on heparin-Sepharose, hydroxyapatite chromatography, gel filtration, and C18 reverse-phase HPLC. Since the purified protein in conjunction with insoluble collagenous bone matrix induced new bone differentiation in vivo we have designated this component osteogenin. The osteogenic potential is specific for osteogenin and is not exhibited by previously isolated growth factors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7109-7113 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 84 |
| Issue number | 20 |
| State | Published - Oct 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
- Genetics
Cite this
Isolation of osteogenin, an extracellular matrix-associated, bone-inductive protein, by heparin affinity chromatography. / Sampath, T. K.; Muthukumaran, N.; Reddi, A Hari.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 20, 10.1987, p. 7109-7113.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of osteogenin, an extracellular matrix-associated, bone-inductive protein, by heparin affinity chromatography.
AU - Sampath, T. K.
AU - Muthukumaran, N.
AU - Reddi, A Hari
PY - 1987/10
Y1 - 1987/10
N2 - Implantation of demineralized diaphyseal bone matrix in subcutaneous sites induces a sequence of events resulting in the local differentiation of endochondral bone. Demineralized bovine bone matrix was dissociatively extracted in 4.0 M guanidine hydrochloride and the bone-inductive proteins were purified greater than 12,000-fold. The purification steps include affinity chromatography on heparin-Sepharose, hydroxyapatite chromatography, gel filtration, and C18 reverse-phase HPLC. Since the purified protein in conjunction with insoluble collagenous bone matrix induced new bone differentiation in vivo we have designated this component osteogenin. The osteogenic potential is specific for osteogenin and is not exhibited by previously isolated growth factors.
AB - Implantation of demineralized diaphyseal bone matrix in subcutaneous sites induces a sequence of events resulting in the local differentiation of endochondral bone. Demineralized bovine bone matrix was dissociatively extracted in 4.0 M guanidine hydrochloride and the bone-inductive proteins were purified greater than 12,000-fold. The purification steps include affinity chromatography on heparin-Sepharose, hydroxyapatite chromatography, gel filtration, and C18 reverse-phase HPLC. Since the purified protein in conjunction with insoluble collagenous bone matrix induced new bone differentiation in vivo we have designated this component osteogenin. The osteogenic potential is specific for osteogenin and is not exhibited by previously isolated growth factors.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023429111&partnerID=8YFLogxK
M3 - Article
C2 - 3478684
AN - SCOPUS:0023429111
VL - 84
SP - 7109
EP - 7113
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 20
ER -