Isolation of myogenic progenitor cell population from human placenta: A pilot study

Sandra K. Kabagambe, Lee Lankford, Priyadarsini Kumar, Y. Julia Chen, Kyle T. Herout, Chelsey J. Lee, Rebecca Stark, Diana L Farmer, Aijun Wang

Research output: Contribution to journalArticle

Abstract

Purpose: The purpose of this study was to demonstrate a method of isolating myogenic progenitor cells from human placenta chorionic villi and to confirm the myogenic characteristics of the isolated cells. Methods: Cells were isolated from chorionic villi of a second trimester male placenta via a combined enzymatic digestion and explant culture. A morphologically distinct subpopulation of elongated and multinucleated cells was identified. This subpopulation was manually passaged from the explant culture, expanded, and analyzed by fluorescence in situ hybridization (FISH) assay, immunocytochemistry, and flow cytometry. Myogenic characteristics including alignment and fusion were tested by growing these cells on aligned polylactic acid microfibrous scaffold in a fusion media composed of 2% horse serum in Dulbecco's modified Eagle medium/high glucose. Results: The expanded subpopulation was uniformly positive for integrin α-7. Presence of Y-chromosome by FISH analysis confirmed chorionic villus origin rather than maternal cell contamination. Isolated cells grew, aligned, and fused on the microfibrous scaffold, and they expressed myogenin, desmin, and MHC confirming their myogenic identity. Conclusion: Myogenic progenitor cells can be isolated from human chorionic villi. This opens the possibility for translational and clinical applications using autologous myogenic cells for possible engraftment in treatment of chest and abdominal wall defects.

Original languageEnglish (US)
JournalJournal of Pediatric Surgery
DOIs
StateAccepted/In press - 2017

Fingerprint

Placenta
Stem Cells
Chorionic Villi
Population
Fluorescence In Situ Hybridization
Myogenin
Eagles
Desmin
Y Chromosome
Second Pregnancy Trimester
Abdominal Wall
Thoracic Wall
Integrins
Horses
Digestion
Flow Cytometry
Immunohistochemistry
Mothers
Glucose
Serum

Keywords

  • Chorionic villi
  • Diaphragmatic hernia
  • Myogenic progenitor cells
  • Placenta
  • Tissue engineering

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

Isolation of myogenic progenitor cell population from human placenta : A pilot study. / Kabagambe, Sandra K.; Lankford, Lee; Kumar, Priyadarsini; Chen, Y. Julia; Herout, Kyle T.; Lee, Chelsey J.; Stark, Rebecca; Farmer, Diana L; Wang, Aijun.

In: Journal of Pediatric Surgery, 2017.

Research output: Contribution to journalArticle

Kabagambe, Sandra K. ; Lankford, Lee ; Kumar, Priyadarsini ; Chen, Y. Julia ; Herout, Kyle T. ; Lee, Chelsey J. ; Stark, Rebecca ; Farmer, Diana L ; Wang, Aijun. / Isolation of myogenic progenitor cell population from human placenta : A pilot study. In: Journal of Pediatric Surgery. 2017.
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AU - Lankford, Lee

AU - Kumar, Priyadarsini

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AU - Herout, Kyle T.

AU - Lee, Chelsey J.

AU - Stark, Rebecca

AU - Farmer, Diana L

AU - Wang, Aijun

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