Isolation of marmoset P450c17 cDNA and gene regulation in vitro

J. Christina Pattison, Alan J Conley, Ian M. Bird

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Marmosets express a fetal zone during adrenal development but fail to produce significant amounts of C19 steroids in adulthood. It is not clear however if P450c17 regulation is different from that in humans/rhesus or the primary sequence is altered. To this end we isolated marmoset and rhesus adrenocortical cells, and treated the cells with known regulators of P450c17 expression for 48 h. P450c17 protein increased with Forskolin (F) treatment, but was marginally inhibited by AII (A) and TPA (T) alone. Combined A + F and T + F dramatically ablated the F response. Cortisol levels (EIA) increased upon F treatment and were inhibited by A and T. Combination of treatments partially inhibited the F-induced response. The protein-coding region of marmoset and rhesus P450c17 cDNAs were then isolated from adrenals using RT-PCR/TA cloning. Marmoset P450c17 shows one amino acid deletion but otherwise shares 90.6% and 91.4% homologies with the human and rhesus cDNA sequences, and 82.4% and 85% homologies with the human and rhesus predicted AA sequences, respectively. Since marmoset adrenocortical cells exhibit similar endocrine function to rhesus, impaired 17,20-lyase activity in the adult marmoset adrenal may in part be due to differences in the primary sequence.

Original languageEnglish (US)
Pages (from-to)737-743
Number of pages7
JournalEndocrine Research
Volume30
Issue number4
DOIs
StatePublished - 2004

Fingerprint

Callithrix
Complementary DNA
Genes
Steroid 17-alpha-Hydroxylase
Colforsin
Open Reading Frames
Hydrocortisone
In Vitro Techniques
Organism Cloning
Therapeutics
Steroids
Amino Acids
Polymerase Chain Reaction
Proteins

Keywords

  • 17 Alpha-hydroxylase
  • Adrenocortical
  • Adult
  • cyt
  • Marmoset

ASJC Scopus subject areas

  • Endocrinology

Cite this

Isolation of marmoset P450c17 cDNA and gene regulation in vitro. / Pattison, J. Christina; Conley, Alan J; Bird, Ian M.

In: Endocrine Research, Vol. 30, No. 4, 2004, p. 737-743.

Research output: Contribution to journalArticle

Pattison, J. Christina ; Conley, Alan J ; Bird, Ian M. / Isolation of marmoset P450c17 cDNA and gene regulation in vitro. In: Endocrine Research. 2004 ; Vol. 30, No. 4. pp. 737-743.
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