Isolation of juvenile hormone esterase and its partial cDNA clone from the beetle, Tenebrio molitor

Beth Ann Thomas, Andrew C. Hinton, Haim Moskowitz, Tonya F. Severson, Bruce D. Hammock

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Juvenile hormone esterase (JHE) plays an essential role in insect development. It is partially responsible for the clearance of juvenile hormone (JH) which regulates various aspects of insect development and reproduction. Because of its role in regulating JH titer, this enzyme has been targeted for development of biologically-based insecticides. JHE was partially purified from the beetle, Tenebrio molitor, using a transition state analog as the affinity ligand. Two forms of JHE were characterized by activity analysis, isoelectric focusing, two-dimensional SDS-PAGE and N- terminal sequence analysis. The esterase is associated with two proteins of sizes 71 and 150 kDa, both of which are active on JH III. A partial cDNA clone for the enzyme was isolated based on the sequence of N-terminal and internal peptides. Its sequence indicates that JHE from T. molitor and Heliothis virescens may have a common origin. (C) 2000 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)529-540
Number of pages12
JournalInsect Biochemistry and Molecular Biology
Volume30
Issue number7
DOIs
StatePublished - Jul 2000

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Keywords

  • Affinity chromatography
  • Esterase
  • Hormones
  • Insects
  • Juvenile hormones
  • Tenebrio molitor

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

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