Isolation of human T-cell lymphotropic virus type 2 from Guaymi Indians in Panama

Michael Dale Lairmore, Steven Jacobson, Fernando Gracia, Barun K. De, Luis Castillo, Mario Larreategui, Beverly D. Roberts, Paul H. Levine, William A. Blattner, Jonathan E. Kaplan

Research output: Contribution to journalArticle

155 Scopus citations

Abstract

Human T-lymphotropic virus type I (HTLV-I) is associated with adult T-cell leukemia/lymphoma and with a chronic degenerative myelopathy. However, another major type of HTLV, HTLV-II, has been isolated only sporadically, and little is known of disease associations, transmission routes, and risk factors for HTLV-II infection. Recent studies indicate that a high percentage of certain groups of i.v. drug users and blood donors are infected with HTLV-II. Seroepidemiologic studies have found an elevated rate of seroreactivity to HTLV among Guaymi Indians from Bocas del Toro Province, Panama. To identify the cause of seroreactivity among this unique population we used HTLV-II-specific polymerase chain reaction techniques to detect HTLV genetic sequences from blood leukocytes of three seropositive Guaymi Indians. The HTLV-II primer-amplified polymerase chain reaction products from two of these subjects were partially sequenced and matched published HTLV-II nucleotide sequences in both p24 gag (94 % of 107 bases) and pol (98 % of 112 bases) regions. A CD4+ T-lymphocyte line established from one of these same subjects produced HTLV-II-specific proteins when tested in antigen-capture and immunoblot assays, as well as mature HTLV particles. The demonstration of HTLV-II infection in this geographically and culturally isolated Central American Indian population without typical risk factors of HTLV infection suggests that HTLV-II infection is endemic in this population and provides an important clue to a potential natural reservoir for this virus.

Original languageEnglish (US)
Pages (from-to)8840-8844
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number22
StatePublished - Nov 1990
Externally publishedYes

Keywords

  • Retrovirus

ASJC Scopus subject areas

  • Genetics
  • General

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    Lairmore, M. D., Jacobson, S., Gracia, F., De, B. K., Castillo, L., Larreategui, M., Roberts, B. D., Levine, P. H., Blattner, W. A., & Kaplan, J. E. (1990). Isolation of human T-cell lymphotropic virus type 2 from Guaymi Indians in Panama. Proceedings of the National Academy of Sciences of the United States of America, 87(22), 8840-8844.