Abstract
We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. Copyright (C) 1998 Federation of European Biochemical Societies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 272-276 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 434 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 4 1998 |
| Externally published | Yes |
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Keywords
- Antimicrobial peptide
- Cryptdin
- Ileal neobladder
- Mucosal immunity
- Phospholipase A2
- Small intestine
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Isolation of human intestinal defensins from ileal neobladder urine. / Porter, Edith Martin; Poles, Michael A.; Lee, Jenny S.; Naitoh, John; Bevins, Charles L; Ganz, Tomas.
In: FEBS Letters, Vol. 434, No. 3, 04.09.1998, p. 272-276.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of human intestinal defensins from ileal neobladder urine
AU - Porter, Edith Martin
AU - Poles, Michael A.
AU - Lee, Jenny S.
AU - Naitoh, John
AU - Bevins, Charles L
AU - Ganz, Tomas
PY - 1998/9/4
Y1 - 1998/9/4
N2 - We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. Copyright (C) 1998 Federation of European Biochemical Societies.
AB - We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. Copyright (C) 1998 Federation of European Biochemical Societies.
KW - Antimicrobial peptide
KW - Cryptdin
KW - Ileal neobladder
KW - Mucosal immunity
KW - Phospholipase A2
KW - Small intestine
UR - http://www.scopus.com/inward/record.url?scp=0032436008&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032436008&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(98)00994-6
DO - 10.1016/S0014-5793(98)00994-6
M3 - Article
C2 - 9742937
AN - SCOPUS:0032436008
VL - 434
SP - 272
EP - 276
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -