Abstract
We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. Copyright (C) 1998 Federation of European Biochemical Societies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 272-276 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 434 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 4 1998 |
| Externally published | Yes |
Keywords
- Antimicrobial peptide
- Cryptdin
- Ileal neobladder
- Mucosal immunity
- Phospholipase A2
- Small intestine
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Cite this
Porter, E. M., Poles, M. A., Lee, J. S., Naitoh, J., Bevins, C. L., & Ganz, T. (1998). Isolation of human intestinal defensins from ileal neobladder urine. FEBS Letters, 434(3), 272-276. https://doi.org/10.1016/S0014-5793(98)00994-6