Isolation of human intestinal defensins from ileal neobladder urine

Edith Martin Porter; Michael A. Poles; Jenny S. Lee; John Naitoh; Charles L Bevins; Tomas Ganz

doi:10.1016/S0014-5793(98)00994-6

Isolation of human intestinal defensins from ileal neobladder urine

Edith Martin Porter, Michael A. Poles, Jenny S. Lee, John Naitoh, Charles L Bevins, Tomas Ganz

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	272-276
Number of pages	5
Journal	FEBS Letters
Volume	434
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00994-6
State	Published - Sep 4 1998
Externally published	Yes

Keywords

Antimicrobial peptide
Cryptdin
Ileal neobladder
Mucosal immunity
Phospholipase A2
Small intestine

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/S0014-5793(98)00994-6

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title = "Isolation of human intestinal defensins from ileal neobladder urine",

abstract = "We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. Copyright (C) 1998 Federation of European Biochemical Societies.",

keywords = "Antimicrobial peptide, Cryptdin, Ileal neobladder, Mucosal immunity, Phospholipase A2, Small intestine",

author = "Porter, {Edith Martin} and Poles, {Michael A.} and Lee, {Jenny S.} and John Naitoh and Bevins, {Charles L} and Tomas Ganz",

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AU - Poles, Michael A.

AU - Lee, Jenny S.

AU - Naitoh, John

AU - Bevins, Charles L

AU - Ganz, Tomas

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N2 - We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. Copyright (C) 1998 Federation of European Biochemical Societies.

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KW - Cryptdin

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KW - Mucosal immunity

KW - Phospholipase A2

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