Abstract
We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. Copyright (C) 1998 Federation of European Biochemical Societies.
Original language | English (US) |
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Pages (from-to) | 272-276 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 434 |
Issue number | 3 |
DOIs | |
State | Published - Sep 4 1998 |
Externally published | Yes |
Keywords
- Antimicrobial peptide
- Cryptdin
- Ileal neobladder
- Mucosal immunity
- Phospholipase A2
- Small intestine
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology