Isolation of human intestinal defensins from ileal neobladder urine

Edith Martin Porter, Michael A. Poles, Jenny S. Lee, John Naitoh, Charles L Bevins, Tomas Ganz

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)272-276
Number of pages5
JournalFEBS Letters
Volume434
Issue number3
DOIs
StatePublished - Sep 4 1998
Externally publishedYes

Keywords

  • Antimicrobial peptide
  • Cryptdin
  • Ileal neobladder
  • Mucosal immunity
  • Phospholipase A2
  • Small intestine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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