Isolation of equine herpesvirus-5 from blood mononuclear cells of a gelding

Stephanie A. Bell, Udeni B R Balasuriya, Robert W. Nordhausen, Nigel J Maclachlan

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Horses are commonly infected by herpesviruses, but isolation of equine herpesvirus-5 (EHV-5) has only infrequently been reported. We describe the isolation and characterization of a strain of EHV-5 from the blood mononuclear cells of a healthy adult horse in California. The virus was initially identified by EHV-5 specific polymerase chain reaction (PCR), and it caused lytic infection of cultured rabbit kidney cells only after repeated serial passage. Virions with characteristic herpesvirus morphology were readily demonstrated in cell culture lysate by transmission electron microscopy. A portion of the glycoprotein B gene of this strain of EHV-5 had 99% identity to the published EHV-5 sequence, and it was clearly distinguishable from other EHV (1-4) by virus-specific PCR assays. Prevalence of EHV-5 infection in a group of young racehorses was estimated at 64% using the EHV-5 specific PCR on nasopharyngeal secretions.

Original languageEnglish (US)
Pages (from-to)472-475
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume18
Issue number5
StatePublished - Sep 2006

Fingerprint

Equid herpesvirus 5
geldings
Herpesviridae
Horses
Blood Cells
blood
cells
polymerase chain reaction
Polymerase Chain Reaction
horses
Equid herpesvirus 1
Equid Herpesvirus 4
viruses
racehorses
Equid Herpesvirus 1
Viruses
Serial Passage
kidney cells
Herpesviridae Infections
virion

Keywords

  • Electron microscopy
  • Equine herpesvirus-5
  • PCR
  • Virus isolation

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Isolation of equine herpesvirus-5 from blood mononuclear cells of a gelding. / Bell, Stephanie A.; Balasuriya, Udeni B R; Nordhausen, Robert W.; Maclachlan, Nigel J.

In: Journal of Veterinary Diagnostic Investigation, Vol. 18, No. 5, 09.2006, p. 472-475.

Research output: Contribution to journalArticle

Bell, Stephanie A. ; Balasuriya, Udeni B R ; Nordhausen, Robert W. ; Maclachlan, Nigel J. / Isolation of equine herpesvirus-5 from blood mononuclear cells of a gelding. In: Journal of Veterinary Diagnostic Investigation. 2006 ; Vol. 18, No. 5. pp. 472-475.
@article{8ebe3e9bbe574231b891e274db6e94e7,
title = "Isolation of equine herpesvirus-5 from blood mononuclear cells of a gelding",
abstract = "Horses are commonly infected by herpesviruses, but isolation of equine herpesvirus-5 (EHV-5) has only infrequently been reported. We describe the isolation and characterization of a strain of EHV-5 from the blood mononuclear cells of a healthy adult horse in California. The virus was initially identified by EHV-5 specific polymerase chain reaction (PCR), and it caused lytic infection of cultured rabbit kidney cells only after repeated serial passage. Virions with characteristic herpesvirus morphology were readily demonstrated in cell culture lysate by transmission electron microscopy. A portion of the glycoprotein B gene of this strain of EHV-5 had 99{\%} identity to the published EHV-5 sequence, and it was clearly distinguishable from other EHV (1-4) by virus-specific PCR assays. Prevalence of EHV-5 infection in a group of young racehorses was estimated at 64{\%} using the EHV-5 specific PCR on nasopharyngeal secretions.",
keywords = "Electron microscopy, Equine herpesvirus-5, PCR, Virus isolation",
author = "Bell, {Stephanie A.} and Balasuriya, {Udeni B R} and Nordhausen, {Robert W.} and Maclachlan, {Nigel J}",
year = "2006",
month = "9",
language = "English (US)",
volume = "18",
pages = "472--475",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "5",

}

TY - JOUR

T1 - Isolation of equine herpesvirus-5 from blood mononuclear cells of a gelding

AU - Bell, Stephanie A.

AU - Balasuriya, Udeni B R

AU - Nordhausen, Robert W.

AU - Maclachlan, Nigel J

PY - 2006/9

Y1 - 2006/9

N2 - Horses are commonly infected by herpesviruses, but isolation of equine herpesvirus-5 (EHV-5) has only infrequently been reported. We describe the isolation and characterization of a strain of EHV-5 from the blood mononuclear cells of a healthy adult horse in California. The virus was initially identified by EHV-5 specific polymerase chain reaction (PCR), and it caused lytic infection of cultured rabbit kidney cells only after repeated serial passage. Virions with characteristic herpesvirus morphology were readily demonstrated in cell culture lysate by transmission electron microscopy. A portion of the glycoprotein B gene of this strain of EHV-5 had 99% identity to the published EHV-5 sequence, and it was clearly distinguishable from other EHV (1-4) by virus-specific PCR assays. Prevalence of EHV-5 infection in a group of young racehorses was estimated at 64% using the EHV-5 specific PCR on nasopharyngeal secretions.

AB - Horses are commonly infected by herpesviruses, but isolation of equine herpesvirus-5 (EHV-5) has only infrequently been reported. We describe the isolation and characterization of a strain of EHV-5 from the blood mononuclear cells of a healthy adult horse in California. The virus was initially identified by EHV-5 specific polymerase chain reaction (PCR), and it caused lytic infection of cultured rabbit kidney cells only after repeated serial passage. Virions with characteristic herpesvirus morphology were readily demonstrated in cell culture lysate by transmission electron microscopy. A portion of the glycoprotein B gene of this strain of EHV-5 had 99% identity to the published EHV-5 sequence, and it was clearly distinguishable from other EHV (1-4) by virus-specific PCR assays. Prevalence of EHV-5 infection in a group of young racehorses was estimated at 64% using the EHV-5 specific PCR on nasopharyngeal secretions.

KW - Electron microscopy

KW - Equine herpesvirus-5

KW - PCR

KW - Virus isolation

UR - http://www.scopus.com/inward/record.url?scp=33749499765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749499765&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 472

EP - 475

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 5

ER -