TY - JOUR
T1 - Isolation of cancer stem cells from adult glioblastoma multiforme
AU - Yuan, Xiangpeng
AU - Curtin, James
AU - Xiong, Yizhi
AU - Liu, Gentao
AU - Wachsmann-Hogiu, Sebastian
AU - Farkas, Daniel L.
AU - Black, Keith L.
AU - Yu, John S.
PY - 2004/12/16
Y1 - 2004/12/16
N2 - Glioblastoma multiforme (GBM) is the most common adult primary brain tumor and is comprised of a heterogeneous population of cells. It is unclear which cells within the tumor mass are responsible for tumor initiation and maintenance. In this study, we report that brain tumor stem cells can be identified from adult GBMs. These tumor stem cells form neurospheres, possess the capacity for self-renewal, express genes associated with neural stem cells (NSCs), generate daughter cells of different phenotypes from one mother cell, and differentiate into the phenotypically diverse populations of cells similar to those present in the initial GBM. Having a distinguishing feature from normal NSCs, these tumor stem cells can reform spheres even after the induction of differentiation. Furthermore, only these tumor stem cells were able to form tumors and generate both neurons and glial cells after in vivo implantation into nude mice. The identification of tumor stem cells within adult GBM may represent a major step forward in understanding the origin and maintenance of GBM and lead to the identification and testing of new therapeutic targets.
AB - Glioblastoma multiforme (GBM) is the most common adult primary brain tumor and is comprised of a heterogeneous population of cells. It is unclear which cells within the tumor mass are responsible for tumor initiation and maintenance. In this study, we report that brain tumor stem cells can be identified from adult GBMs. These tumor stem cells form neurospheres, possess the capacity for self-renewal, express genes associated with neural stem cells (NSCs), generate daughter cells of different phenotypes from one mother cell, and differentiate into the phenotypically diverse populations of cells similar to those present in the initial GBM. Having a distinguishing feature from normal NSCs, these tumor stem cells can reform spheres even after the induction of differentiation. Furthermore, only these tumor stem cells were able to form tumors and generate both neurons and glial cells after in vivo implantation into nude mice. The identification of tumor stem cells within adult GBM may represent a major step forward in understanding the origin and maintenance of GBM and lead to the identification and testing of new therapeutic targets.
KW - Adult brain tumors
KW - Cancer stem cells
KW - Glioblastoma multiforme
KW - Glioblastoma spheres
KW - In vivo implantation
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U2 - 10.1038/sj.onc.1208311
DO - 10.1038/sj.onc.1208311
M3 - Article
C2 - 15558011
AN - SCOPUS:11244293572
VL - 23
SP - 9392
EP - 9400
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 58
ER -