Isolation of bovine herpesvirus-3 from African buffaloes (Syncerus caffer).

P. B. Rossiter, I. D. Gumm, D. A. Stagg, Patricia A Conrad, S. Mukolwe, F. G. Davies, H. White

Research output: Contribution to journalArticle

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Abstract

Eleven virus isolations were made from the blood of 45 free living healthy African buffaloes by long term cocultivation of their leucocytes with bovine thymus or spleen cells. The isolates were indistinguishable from each other or from herpesviruses isolated from a severely ill buffalo calf and from a dead buffalo. These viruses possessed the characteristics of the bovine herpesvirus-3 (BHV-3) group and were indistinguishable by serology and restriction endonuclease analysis from the BHV-3 type strains Movar 33/63 and DN599. There was a 93.6 per cent prevalence of indirect immunofluorescent antibody to BHV-3 in the sera of 94 buffaloes in the sample population. No clinical signs or viraemia were detected in five cattle inoculated with 10(8.7) log10 TCID50 of the isolate from the sick buffalo calf. Two of three cattle hyperimmunised with this virus resisted challenge with malignant catarrhal fever herpesvirus, which proved fatal for the other immunised animal and for three control cattle.

Original languageEnglish (US)
Pages (from-to)337-343
Number of pages7
JournalResearch in Veterinary Science
Volume46
Issue number3
StatePublished - May 1989
Externally publishedYes

Fingerprint

Syncerus caffer
Herpesviridae
Buffaloes
cattle
buffaloes
Malignant Catarrh
Viruses
viruses
calves
malignant catarrhal fever
restriction mapping
Viremia
viremia
DNA Restriction Enzymes
Serology
splenocytes
Coculture Techniques
blood serum
Thymus Gland
leukocytes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Rossiter, P. B., Gumm, I. D., Stagg, D. A., Conrad, P. A., Mukolwe, S., Davies, F. G., & White, H. (1989). Isolation of bovine herpesvirus-3 from African buffaloes (Syncerus caffer). Research in Veterinary Science, 46(3), 337-343.

Isolation of bovine herpesvirus-3 from African buffaloes (Syncerus caffer). / Rossiter, P. B.; Gumm, I. D.; Stagg, D. A.; Conrad, Patricia A; Mukolwe, S.; Davies, F. G.; White, H.

In: Research in Veterinary Science, Vol. 46, No. 3, 05.1989, p. 337-343.

Research output: Contribution to journalArticle

Rossiter, PB, Gumm, ID, Stagg, DA, Conrad, PA, Mukolwe, S, Davies, FG & White, H 1989, 'Isolation of bovine herpesvirus-3 from African buffaloes (Syncerus caffer).', Research in Veterinary Science, vol. 46, no. 3, pp. 337-343.
Rossiter PB, Gumm ID, Stagg DA, Conrad PA, Mukolwe S, Davies FG et al. Isolation of bovine herpesvirus-3 from African buffaloes (Syncerus caffer). Research in Veterinary Science. 1989 May;46(3):337-343.
Rossiter, P. B. ; Gumm, I. D. ; Stagg, D. A. ; Conrad, Patricia A ; Mukolwe, S. ; Davies, F. G. ; White, H. / Isolation of bovine herpesvirus-3 from African buffaloes (Syncerus caffer). In: Research in Veterinary Science. 1989 ; Vol. 46, No. 3. pp. 337-343.
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