Abstract
A technique is described by which the vaccine strain of bluetongue virus, (BTVV) may be isolated from infected fetal, neonatal, and adult sheep tissues utilizing tissue culture. The data from these studies provides evidence that 1) BTVV can be readily isolated from infected fetal and newborn tissues by tissue culture, 2) mild treatment of tissues and utilization of lysed cells as inoculum provides an effective means of recoverng vaccine virus, 3) BTVV can be isolated with equal efficacy from mononuclear fractions and from erythrocyte granulocyte fractions of veremic blood, and 5) the brain of fetal lambs and the spleen and liver of neonatal lambs appear to be the tissues from which vaccine virus can be consistently isolated.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 65-71 |
| Number of pages | 7 |
| Journal | CORNELL VET. |
| Volume | 67 |
| Issue number | 1 |
| State | Published - Jan 1 1977 |
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ASJC Scopus subject areas
- Medicine(all)
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Isolation of bluetongue vaccine virus from infected sheep by direct inoculation onto tissue culture. / Sawyer, M. M.; Osburn, Bennie.
In: CORNELL VET., Vol. 67, No. 1, 01.01.1977, p. 65-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of bluetongue vaccine virus from infected sheep by direct inoculation onto tissue culture
AU - Sawyer, M. M.
AU - Osburn, Bennie
PY - 1977/1/1
Y1 - 1977/1/1
N2 - A technique is described by which the vaccine strain of bluetongue virus, (BTVV) may be isolated from infected fetal, neonatal, and adult sheep tissues utilizing tissue culture. The data from these studies provides evidence that 1) BTVV can be readily isolated from infected fetal and newborn tissues by tissue culture, 2) mild treatment of tissues and utilization of lysed cells as inoculum provides an effective means of recoverng vaccine virus, 3) BTVV can be isolated with equal efficacy from mononuclear fractions and from erythrocyte granulocyte fractions of veremic blood, and 5) the brain of fetal lambs and the spleen and liver of neonatal lambs appear to be the tissues from which vaccine virus can be consistently isolated.
AB - A technique is described by which the vaccine strain of bluetongue virus, (BTVV) may be isolated from infected fetal, neonatal, and adult sheep tissues utilizing tissue culture. The data from these studies provides evidence that 1) BTVV can be readily isolated from infected fetal and newborn tissues by tissue culture, 2) mild treatment of tissues and utilization of lysed cells as inoculum provides an effective means of recoverng vaccine virus, 3) BTVV can be isolated with equal efficacy from mononuclear fractions and from erythrocyte granulocyte fractions of veremic blood, and 5) the brain of fetal lambs and the spleen and liver of neonatal lambs appear to be the tissues from which vaccine virus can be consistently isolated.
UR - http://www.scopus.com/inward/record.url?scp=0017330485&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017330485&partnerID=8YFLogxK
M3 - Article
C2 - 188594
AN - SCOPUS:0017330485
VL - 67
SP - 65
EP - 71
JO - The Cornell veterinarian
JF - The Cornell veterinarian
SN - 0010-8901
IS - 1
ER -