Isolation of a strain of Agrobacterium tumefaciens (Rhizobium radiobacter) utilizing methylene urea (ureaformaldehyde) as nitrogen source

Marja E. Koivunen, Christophe Morisseau, William R. Horwath, Bruce D. Hammock

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Methylene ureas (MU) are slow-release nitrogen fertilizers degraded in soil by microbial enzymatic activity. Improved utilization of MU in agricultural production requires more knowledge about the organisms and enzymes responsible for its degradation. A Gram-negative, MU-degrading organism was isolated from a soil in Sacramento Valley, California. The bacterium was identified as Agrobacterium tumefaciens (recently also known as Rhizobium radiobacter) using both genotypic and phenotypic characterization. The pathogenic nature of the organism was confirmed by a bioassay on carrot disks. The MU-hydrolyzing enzyme (MUase) was intracellular and was induced by using MU as a sole source of nitrogen. The bacterial growth was optimized in NH4Cl, urea, or peptone, whereas the production and specific activity of MUase were maximized with either NH4Cl or urea as a nitrogen source. The result has a practical significance, demonstrating a potential to select for this plant pathogen in soils fertilized with MU.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalCanadian Journal of Microbiology
Volume50
Issue number3
DOIs
StatePublished - Mar 2004

Fingerprint

Agrobacterium tumefaciens
Urea
Nitrogen
Soil
Soils
Nitrogen fertilizers
Peptones
Daucus carota
Bioassay
Fertilizers
Pathogens
Enzymes
Biological Assay
Bacteria
Degradation
Growth

Keywords

  • Agrobacterium tumefaciens
  • Isolation
  • Methylene urea
  • Nitrogen
  • Rhizobium radiobacter
  • Slow-release fertilizer
  • Soil
  • Ureaformaldehyde

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Immunology
  • Microbiology

Cite this

Isolation of a strain of Agrobacterium tumefaciens (Rhizobium radiobacter) utilizing methylene urea (ureaformaldehyde) as nitrogen source. / Koivunen, Marja E.; Morisseau, Christophe; Horwath, William R.; Hammock, Bruce D.

In: Canadian Journal of Microbiology, Vol. 50, No. 3, 03.2004, p. 167-174.

Research output: Contribution to journalArticle

Koivunen, Marja E. ; Morisseau, Christophe ; Horwath, William R. ; Hammock, Bruce D. / Isolation of a strain of Agrobacterium tumefaciens (Rhizobium radiobacter) utilizing methylene urea (ureaformaldehyde) as nitrogen source. In: Canadian Journal of Microbiology. 2004 ; Vol. 50, No. 3. pp. 167-174.
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