Isolation, characterization, and stability of discretely-sized nanolipoprotein particles assembled with apolipophorin-III

Nicholas O Fischer, Craig D. Blanchette, Brent W. Segelke, Michele Corzett, Brett A. Chromy, Edward A. Kuhn, Graham Bench, Paul D. Hoeprich

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Nanolipoprotein particles (NLPs) are discoidal, nanometer-sized particles comprised of self-assembled phospholipid membranes and apolipoproteins. NLPs assembled with human apolipoproteins have been used for myriad biotechnology applications, including membrane protein solubilization, drug delivery, and diagnostic imaging. To expand the repertoire of lipoproteins for these applications, insect apolipophorin-III (apoLp-III) was evaluated for the ability to form discretely-sized, homogeneous, and stable NLPs. Methodology: Four NLP populations distinct with regards to particle diameters (ranging in size from 10 nm to > 25 nm) and lipid-to-apoLp-III ratios were readily isolated to high purity by size exclusion chromatography. Remodeling of the purified NLP species over time at 4°C was monitored by native gel electrophoresis, size exclusion chromatography, and atomic force microscopy. Purified 20 nm NLPs displayed no remodeling and remained stable for over 1 year. Purified NLPs with 10 nm and 15 nm diameters ultimately remodeled into 20 nm NLPs over a period of months. Intra-particle chemical cross-linking of apoLp-III stabilized NLPs of all sizes. Conclusions: ApoLp-III-based NLPs can be readily prepared, purified, characterized, and stabilized, suggesting their utility for biotechnological applications.

Original languageEnglish (US)
Article numbere11643
JournalPLoS One
Volume5
Issue number7
DOIs
StatePublished - Jul 19 2010
Externally publishedYes

Fingerprint

apolipophorin
Size exclusion chromatography
Apolipoproteins
Gel Chromatography
Atomic Force Microscopy
Diagnostic Imaging
Biotechnology
Electrophoresis
Drug delivery
Particle Size
Lipoproteins
Insects
Atomic force microscopy
Phospholipids
Membrane Proteins
Gels
Membranes
Lipids
Imaging techniques
apolipoproteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Fischer, N. O., Blanchette, C. D., Segelke, B. W., Corzett, M., Chromy, B. A., Kuhn, E. A., ... Hoeprich, P. D. (2010). Isolation, characterization, and stability of discretely-sized nanolipoprotein particles assembled with apolipophorin-III. PLoS One, 5(7), [e11643]. https://doi.org/10.1371/journal.pone.0011643

Isolation, characterization, and stability of discretely-sized nanolipoprotein particles assembled with apolipophorin-III. / Fischer, Nicholas O; Blanchette, Craig D.; Segelke, Brent W.; Corzett, Michele; Chromy, Brett A.; Kuhn, Edward A.; Bench, Graham; Hoeprich, Paul D.

In: PLoS One, Vol. 5, No. 7, e11643, 19.07.2010.

Research output: Contribution to journalArticle

Fischer, NO, Blanchette, CD, Segelke, BW, Corzett, M, Chromy, BA, Kuhn, EA, Bench, G & Hoeprich, PD 2010, 'Isolation, characterization, and stability of discretely-sized nanolipoprotein particles assembled with apolipophorin-III', PLoS One, vol. 5, no. 7, e11643. https://doi.org/10.1371/journal.pone.0011643
Fischer, Nicholas O ; Blanchette, Craig D. ; Segelke, Brent W. ; Corzett, Michele ; Chromy, Brett A. ; Kuhn, Edward A. ; Bench, Graham ; Hoeprich, Paul D. / Isolation, characterization, and stability of discretely-sized nanolipoprotein particles assembled with apolipophorin-III. In: PLoS One. 2010 ; Vol. 5, No. 7.
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