Abstract
Tyrosine hydroxylase (TH, tyrosine 3-monooxygenase; EC 1.14.16.2) activity in curde extracts of rat pheochromocytoma, rat brain, and bovine adrenal medulla can be immunoprecipitated in an indirect assay by monoclonal antibodies prepared against partially purified rat pheochromocytoma TH. One of these monoclonal antibodies, TH-2D8-2, can be used for immunocytochemical localization of TH in cell bodies, dendrites, and axons in catecholaminergic neurons (e.g., cells in the substantia nigra) of rat brain and in the cell body, neurites, and growth cones of rat pheochromocytoma cells after treatment with nerve growth factor. When linked to Affi-gel 10, this monoclonal antibody can also be used for immunoaffinity purification of rat and bovine TH. These results suggest that TH-2D8-2 is a valuable reagent with which to investigate the localization, physiological regulation, and function of this important enzyme.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 316-325 |
| Number of pages | 10 |
| Journal | Journal of Neuroscience Research |
| Volume | 23 |
| Issue number | 3 |
| State | Published - 1989 |
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ASJC Scopus subject areas
- Neuroscience(all)
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Isolation, characterization, and application of monoclonal antibodies to rat tyrosine hydroxylase. / Kwan, S. W.; Patel, N. T.; Vulliet, Philip R; Hall, F. L.; Denney, R. M.; Shen, R. S.; Westlund, K. N.; Abell, C. W.
In: Journal of Neuroscience Research, Vol. 23, No. 3, 1989, p. 316-325.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation, characterization, and application of monoclonal antibodies to rat tyrosine hydroxylase
AU - Kwan, S. W.
AU - Patel, N. T.
AU - Vulliet, Philip R
AU - Hall, F. L.
AU - Denney, R. M.
AU - Shen, R. S.
AU - Westlund, K. N.
AU - Abell, C. W.
PY - 1989
Y1 - 1989
N2 - Tyrosine hydroxylase (TH, tyrosine 3-monooxygenase; EC 1.14.16.2) activity in curde extracts of rat pheochromocytoma, rat brain, and bovine adrenal medulla can be immunoprecipitated in an indirect assay by monoclonal antibodies prepared against partially purified rat pheochromocytoma TH. One of these monoclonal antibodies, TH-2D8-2, can be used for immunocytochemical localization of TH in cell bodies, dendrites, and axons in catecholaminergic neurons (e.g., cells in the substantia nigra) of rat brain and in the cell body, neurites, and growth cones of rat pheochromocytoma cells after treatment with nerve growth factor. When linked to Affi-gel 10, this monoclonal antibody can also be used for immunoaffinity purification of rat and bovine TH. These results suggest that TH-2D8-2 is a valuable reagent with which to investigate the localization, physiological regulation, and function of this important enzyme.
AB - Tyrosine hydroxylase (TH, tyrosine 3-monooxygenase; EC 1.14.16.2) activity in curde extracts of rat pheochromocytoma, rat brain, and bovine adrenal medulla can be immunoprecipitated in an indirect assay by monoclonal antibodies prepared against partially purified rat pheochromocytoma TH. One of these monoclonal antibodies, TH-2D8-2, can be used for immunocytochemical localization of TH in cell bodies, dendrites, and axons in catecholaminergic neurons (e.g., cells in the substantia nigra) of rat brain and in the cell body, neurites, and growth cones of rat pheochromocytoma cells after treatment with nerve growth factor. When linked to Affi-gel 10, this monoclonal antibody can also be used for immunoaffinity purification of rat and bovine TH. These results suggest that TH-2D8-2 is a valuable reagent with which to investigate the localization, physiological regulation, and function of this important enzyme.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024371040&partnerID=8YFLogxK
M3 - Article
C2 - 2570162
AN - SCOPUS:0024371040
VL - 23
SP - 316
EP - 325
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 3
ER -