Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

Margaret L. Palmer; Mary Alice Raker; Poindexter J. Kennedy; Jon W. Young; Wayne M. Barnes; Raymond L. Rodriguez; Harry F. Noller

doi:10.1007/BF00268279

Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

Margaret L. Palmer, Mary Alice Raker, Poindexter J. Kennedy, Jon W. Young, Wayne M. Barnes, Raymond L. Rodriguez, Harry F. Noller

Molecular and Cellular Biology

Research output: Contribution to journal › Article

7 Scopus citations

Abstract

Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.

Original language	English (US)
Pages (from-to)	171-178
Number of pages	8
Journal	MGG Molecular & General Genetics
Volume	172
Issue number	2
DOIs	https://doi.org/10.1007/BF00268279
State	Published - Jan 1979

ASJC Scopus subject areas

Genetics

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10.1007/BF00268279

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Palmer, M. L., Raker, M. A., Kennedy, P. J., Young, J. W., Barnes, W. M., Rodriguez, R. L., & Noller, H. F. (1979). Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli. MGG Molecular & General Genetics, 172(2), 171-178. https://doi.org/10.1007/BF00268279

Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli. / Palmer, Margaret L.; Raker, Mary Alice; Kennedy, Poindexter J.; Young, Jon W.; Barnes, Wayne M.; Rodriguez, Raymond L.; Noller, Harry F.

In: MGG Molecular & General Genetics, Vol. 172, No. 2, 01.1979, p. 171-178.

Research output: Contribution to journal › Article

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abstract = "Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.",

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