Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

Margaret L. Palmer, Mary Alice Raker, Poindexter J. Kennedy, Jon W. Young, Wayne M. Barnes, Raymond L. Rodriguez, Harry F. Noller

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.

Original languageEnglish (US)
Pages (from-to)171-178
Number of pages8
JournalMGG Molecular & General Genetics
Volume172
Issue number2
DOIs
StatePublished - Jan 1979

ASJC Scopus subject areas

  • Genetics

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    Palmer, M. L., Raker, M. A., Kennedy, P. J., Young, J. W., Barnes, W. M., Rodriguez, R. L., & Noller, H. F. (1979). Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli. MGG Molecular & General Genetics, 172(2), 171-178. https://doi.org/10.1007/BF00268279