TY - JOUR
T1 - Isolation and potential immunological characterization of TPSGLVY, a novel bursal septpeptide isolated from the bursa of fabricius
AU - Feng, Xiuli
AU - Su, Xiaodong
AU - Wang, Fangquan
AU - Wei, Jianchao
AU - Wang, Fengjuan
AU - Cao, Ruibing
AU - Zhou, Bin
AU - Mao, Xiang
AU - Zheng, Qisheng
AU - Chen, Puyan
PY - 2010/1/1
Y1 - 2010/1/1
N2 - The bursa of Fabricius is central immune organ unique to birds, and the extract is immunocompetent in stimulating B cell differentiation and enhancing antibody production. However, except for bursin, the active peptides from the bursa of Fabricius are little reported. In the paper, a novel bursal septpeptide (BSP-II) with the amino acids sequence of TPSGLVY was identified and similar to the MGC53864 protein of Gallus gallus.We investigated the effects of BSP-II on the immune response in terms of the antibodies titers (IgG1 and IgG2α), the levels of interferon-γ and interleukin-4 cytokines, spleen cell lymphocyte proliferation, and the T-lymphocyte subtype composition. It was noteworthy that BSP-II potentiates the Th1 and Th2-type immune responses in dose-dependent manner. BSP-II had specific enhancing effects on the hybridoma SP2/0 cell proliferation at two different serum concentrations (20% and 5%), but had no connection with the dose of BSP-II. The antibody secreting level of hybridoma SP2/0 cells rose in 5% and 20% serum when the concentrations of BSP-II increased. Also, BSP-II had effect on the viabilities of tumor cells (Hela and SP2/0). All the results indicated that BSP-II was able to significantly induce various immune responses and involved in the cell viability of different tumor cell lines. Our observations implied that BSPII might be a novel biological active factor from the bursa of Fabricius with immunomodulatory activities. Crown
AB - The bursa of Fabricius is central immune organ unique to birds, and the extract is immunocompetent in stimulating B cell differentiation and enhancing antibody production. However, except for bursin, the active peptides from the bursa of Fabricius are little reported. In the paper, a novel bursal septpeptide (BSP-II) with the amino acids sequence of TPSGLVY was identified and similar to the MGC53864 protein of Gallus gallus.We investigated the effects of BSP-II on the immune response in terms of the antibodies titers (IgG1 and IgG2α), the levels of interferon-γ and interleukin-4 cytokines, spleen cell lymphocyte proliferation, and the T-lymphocyte subtype composition. It was noteworthy that BSP-II potentiates the Th1 and Th2-type immune responses in dose-dependent manner. BSP-II had specific enhancing effects on the hybridoma SP2/0 cell proliferation at two different serum concentrations (20% and 5%), but had no connection with the dose of BSP-II. The antibody secreting level of hybridoma SP2/0 cells rose in 5% and 20% serum when the concentrations of BSP-II increased. Also, BSP-II had effect on the viabilities of tumor cells (Hela and SP2/0). All the results indicated that BSP-II was able to significantly induce various immune responses and involved in the cell viability of different tumor cell lines. Our observations implied that BSPII might be a novel biological active factor from the bursa of Fabricius with immunomodulatory activities. Crown
KW - Hybridoma SP2/0
KW - Immune response
KW - Second bursal septpeptide (BSP-II)
KW - Tumor cell viability
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U2 - 10.1016/j.peptides.2010.05.007
DO - 10.1016/j.peptides.2010.05.007
M3 - Article
C2 - 20493914
AN - SCOPUS:78249254849
VL - 31
SP - 1562
EP - 1568
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 8
ER -