Isolation and potential immunological characterization of TPSGLVY, a novel bursal septpeptide isolated from the bursa of fabricius

Xiuli Feng, Xiaodong Su, Fangquan Wang, Jianchao Wei, Fengjuan Wang, Ruibing Cao, Bin Zhou, Xiang Mao, Qisheng Zheng, Puyan Chen

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The bursa of Fabricius is central immune organ unique to birds, and the extract is immunocompetent in stimulating B cell differentiation and enhancing antibody production. However, except for bursin, the active peptides from the bursa of Fabricius are little reported. In the paper, a novel bursal septpeptide (BSP-II) with the amino acids sequence of TPSGLVY was identified and similar to the MGC53864 protein of Gallus gallus.We investigated the effects of BSP-II on the immune response in terms of the antibodies titers (IgG1 and IgG2α), the levels of interferon-γ and interleukin-4 cytokines, spleen cell lymphocyte proliferation, and the T-lymphocyte subtype composition. It was noteworthy that BSP-II potentiates the Th1 and Th2-type immune responses in dose-dependent manner. BSP-II had specific enhancing effects on the hybridoma SP2/0 cell proliferation at two different serum concentrations (20% and 5%), but had no connection with the dose of BSP-II. The antibody secreting level of hybridoma SP2/0 cells rose in 5% and 20% serum when the concentrations of BSP-II increased. Also, BSP-II had effect on the viabilities of tumor cells (Hela and SP2/0). All the results indicated that BSP-II was able to significantly induce various immune responses and involved in the cell viability of different tumor cell lines. Our observations implied that BSPII might be a novel biological active factor from the bursa of Fabricius with immunomodulatory activities. Crown

Original languageEnglish (US)
Pages (from-to)1562-1568
Number of pages7
JournalPeptides
Volume31
Issue number8
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

Keywords

  • Hybridoma SP2/0
  • Immune response
  • Second bursal septpeptide (BSP-II)
  • Tumor cell viability

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Isolation and potential immunological characterization of TPSGLVY, a novel bursal septpeptide isolated from the bursa of fabricius'. Together they form a unique fingerprint.

  • Cite this