Isolation and partial characterization of a lentivirus from talapoin monkeys (Myopithecus talapoin)

Albert D M E Osterhaus, Niels C Pedersen, Geert Van Amerongen, Maarten T. Frankenhuis, Marta Marthas, Elizabeth Reay, Timothy M. Rose, Joko Pamungkas, Marnix L. Bosch

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Abstract

We have identified a novel lentivirus prevalent in talapoin monkeys (Myopithecus talapoin), extending previous observations of human immunodeficiency virus-1 cross-reactive antibodies in the serum of these monkeys. We obtained a virus isolate from one of three seropositive monkeys initially available to us. The virus was tentatively named simian immunodeficiency virus from talapoin monkeys (SIVtal). Despite the difficulty of isolating this virus, it was readily passed between monkeys in captivity through unknown routes of transmission. The virus could be propagated for short terms in peripheral blood mononuclear cells of talapoin monkeys but not in human peripheral blood mononuclear cells or human T cell lines. The propagated virus was used to infect a naive talapoin monkey, four rhesus macaques (M. mulatta), and two cynomolgus macaques (M. fascicularis). All animals seroconverted and virus could be reisolated during a short period after experimental infection. A survey of SIVtal-infected captive talapoin monkeys revealed a relative decrease in CD4+ cell numbers in chronically (>2 years) infected animals. No other signs of immunodeficiency were observed in any of the infected animals. PCR amplification followed by DNA sequencing of two fragments of the polymerase gene revealed that SIVtal is different from the presently known lentiviruses and perhaps most related to the SIV from Sykes monkeys.

Original languageEnglish (US)
Pages (from-to)116-124
Number of pages9
JournalVirology
Volume260
Issue number1
DOIs
StatePublished - Jul 20 1999

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Cercopithecidae
Lentivirus
Viruses
Simian Immunodeficiency Virus
Haplorhini
Blood Cells
Macaca
Macaca mulatta
DNA Sequence Analysis
HIV-1
Cell Count
T-Lymphocytes
Cell Line
Polymerase Chain Reaction
Antibodies
Infection
Serum

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Osterhaus, A. D. M. E., Pedersen, N. C., Van Amerongen, G., Frankenhuis, M. T., Marthas, M., Reay, E., ... Bosch, M. L. (1999). Isolation and partial characterization of a lentivirus from talapoin monkeys (Myopithecus talapoin). Virology, 260(1), 116-124. https://doi.org/10.1006/viro.1999.9794

Isolation and partial characterization of a lentivirus from talapoin monkeys (Myopithecus talapoin). / Osterhaus, Albert D M E; Pedersen, Niels C; Van Amerongen, Geert; Frankenhuis, Maarten T.; Marthas, Marta; Reay, Elizabeth; Rose, Timothy M.; Pamungkas, Joko; Bosch, Marnix L.

In: Virology, Vol. 260, No. 1, 20.07.1999, p. 116-124.

Research output: Contribution to journalArticle

Osterhaus, ADME, Pedersen, NC, Van Amerongen, G, Frankenhuis, MT, Marthas, M, Reay, E, Rose, TM, Pamungkas, J & Bosch, ML 1999, 'Isolation and partial characterization of a lentivirus from talapoin monkeys (Myopithecus talapoin)', Virology, vol. 260, no. 1, pp. 116-124. https://doi.org/10.1006/viro.1999.9794
Osterhaus, Albert D M E ; Pedersen, Niels C ; Van Amerongen, Geert ; Frankenhuis, Maarten T. ; Marthas, Marta ; Reay, Elizabeth ; Rose, Timothy M. ; Pamungkas, Joko ; Bosch, Marnix L. / Isolation and partial characterization of a lentivirus from talapoin monkeys (Myopithecus talapoin). In: Virology. 1999 ; Vol. 260, No. 1. pp. 116-124.
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