Isolation and characterization of the hisG and hisD genes of Klebsiella pneumoniae

Raymond L. Rodriguez, Robert W. West, Robert C. Tait, Jesse M. Jaynes, K. T. Shanmugam

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The hisG and hisD genes of the histidine biosynthesis operon of Klebsiella pneumoniae have been cloned in pBR322 and identified by complementation analysis in K. pneumoniae and E. coli. DNA sequence analysis suggests that the genes are functionally expressed in the absence of their complete regulatory sequences.

Original languageEnglish (US)
Pages (from-to)317-320
Number of pages4
JournalGene
Volume16
Issue number1-3
DOIs
StatePublished - 1981

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Keywords

  • cloning vector pBR322
  • DNA sequencing
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Rodriguez, R. L., West, R. W., Tait, R. C., Jaynes, J. M., & Shanmugam, K. T. (1981). Isolation and characterization of the hisG and hisD genes of Klebsiella pneumoniae. Gene, 16(1-3), 317-320. https://doi.org/10.1016/0378-1119(81)90087-1