Isolation and characterization of the hisG and hisD genes of Klebsiella pneumoniae

Raymond L. Rodriguez; Robert W. West; Robert C. Tait; Jesse M. Jaynes; K. T. Shanmugam

doi:10.1016/0378-1119(81)90087-1

Isolation and characterization of the hisG and hisD genes of Klebsiella pneumoniae

Raymond L. Rodriguez, Robert W. West, Robert C. Tait, Jesse M. Jaynes, K. T. Shanmugam

Molecular and Cellular Biology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The hisG and hisD genes of the histidine biosynthesis operon of Klebsiella pneumoniae have been cloned in pBR322 and identified by complementation analysis in K. pneumoniae and E. coli. DNA sequence analysis suggests that the genes are functionally expressed in the absence of their complete regulatory sequences.

Original language	English (US)
Pages (from-to)	317-320
Number of pages	4
Journal	Gene
Volume	16
Issue number	1-3
DOIs	https://doi.org/10.1016/0378-1119(81)90087-1
State	Published - 1981

Keywords

cloning vector pBR322
DNA sequencing
Recombinant DNA

ASJC Scopus subject areas

Genetics

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title = "Isolation and characterization of the hisG and hisD genes of Klebsiella pneumoniae",

abstract = "The hisG and hisD genes of the histidine biosynthesis operon of Klebsiella pneumoniae have been cloned in pBR322 and identified by complementation analysis in K. pneumoniae and E. coli. DNA sequence analysis suggests that the genes are functionally expressed in the absence of their complete regulatory sequences.",

keywords = "cloning vector pBR322, DNA sequencing, Recombinant DNA",

author = "Rodriguez, {Raymond L.} and West, {Robert W.} and Tait, {Robert C.} and Jaynes, {Jesse M.} and Shanmugam, {K. T.}",

year = "1981",

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pages = "317--320",

journal = "Gene",

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T1 - Isolation and characterization of the hisG and hisD genes of Klebsiella pneumoniae

AU - Rodriguez, Raymond L.

AU - West, Robert W.

AU - Tait, Robert C.

AU - Jaynes, Jesse M.

AU - Shanmugam, K. T.

PY - 1981

Y1 - 1981

N2 - The hisG and hisD genes of the histidine biosynthesis operon of Klebsiella pneumoniae have been cloned in pBR322 and identified by complementation analysis in K. pneumoniae and E. coli. DNA sequence analysis suggests that the genes are functionally expressed in the absence of their complete regulatory sequences.

AB - The hisG and hisD genes of the histidine biosynthesis operon of Klebsiella pneumoniae have been cloned in pBR322 and identified by complementation analysis in K. pneumoniae and E. coli. DNA sequence analysis suggests that the genes are functionally expressed in the absence of their complete regulatory sequences.

KW - cloning vector pBR322

KW - DNA sequencing

KW - Recombinant DNA

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AN - SCOPUS:0019767288

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JO - Gene

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