Isolation and characterization of soluble boron complexes in higher plants: The mechanism of phloem mobility of boron

H. Hu, S. G. Penn, Carlito B Lebrilla, P. H. Brown

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Abstract

Boron (B) polyol complexes have been isolated and characterized from the phloem sap of celery (Apium graveolens L.) and the extrafloral nectar of peach (Prunuspersica L.). In celery the direct analysis of untreated phloem sap by matrix-assisted laser desorption-Fourier transform mass spectrometry, with verification by high-performance liquid chromagraphy and gas chromatography-mass spectrometry, revealed that B is present in the phloem as the mannitol-B-mannitol complex. Molecular modeling further predicted that this complex is present in the 3,4 3',4' bis-mannitol configuration. In the extrafloral nectar of peach, B was present as a mixture of sorbitol-B-sorbitol, fructose-B-fructose, or sorbitol-B-fructose. To our knowledge, these findings represent the first successful isolation and characterization of soluble B complexes from higher plants and provide a mechanistic explanation for the observed phloem B mobility in these species.

Original languageEnglish (US)
Pages (from-to)649-655
Number of pages7
JournalPlant Physiology
Volume113
Issue number2
StatePublished - Feb 1997

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ASJC Scopus subject areas

  • Plant Science

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