Isolation and characterization of Sarcocystis from brain tissue of a free-living southern sea otter (Enhydra lutris nereis) with fatal meningoencephalitis

Melissa A. Miller, Paul R. Crosbie, Karen Sverlow, Krista Hanni, Bradd C. Barr, Nancy Kock, Michael J. Murray, Linda J Lowenstine, Patricia A Conrad

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A protozoan was isolated in cell culture from the brain of a free-ranging sea otter with fatal meningoencephalitis. The biological history of this otter, a study animal being monitored via an intraperitoneal radio transmitter, is summarized. Histologically, protozoal parasites were associated with areas of brain inflammation and necrosis in the cerebrum and cerebellum. Morphology and measurements of fixed, Giemsa-stained protozoal zoites growing on coverslips were consistent with Sarcocystis. These parasites reacted only with polyclonal antisera raised against S. neurona on immunohistochemistry. Cell culture-derived zoites reacted strongly with polyclonal antiserum to S. neurona on indirect fluorescent antibody tests. Amplification of portions of the 18S ribosomal DNA and the adjacent first internal transcribed spacer were performed. The resulting sequences were compared with published sequences from similar apicomplexan protozoa. This isolate (SO SN1), was indistinguishable from S. neurona, based on parasite morphology, antigenic reactivity and molecular characterization.

Original languageEnglish (US)
Pages (from-to)252-257
Number of pages6
JournalParasitology Research
Volume87
Issue number3
DOIs
StatePublished - 2001

Fingerprint

Otters
Sarcocystis
Meningoencephalitis
meningoencephalitis
Parasites
brain
parasites
Protozoa
antiserum
Immune Sera
Brain
cell culture
Cell Culture Techniques
Sarcocystis neurona
Enhydra lutris
cerebrum
radio transmitters
Cerebrum
cerebellum
Encephalitis

ASJC Scopus subject areas

  • Parasitology

Cite this

Isolation and characterization of Sarcocystis from brain tissue of a free-living southern sea otter (Enhydra lutris nereis) with fatal meningoencephalitis. / Miller, Melissa A.; Crosbie, Paul R.; Sverlow, Karen; Hanni, Krista; Barr, Bradd C.; Kock, Nancy; Murray, Michael J.; Lowenstine, Linda J; Conrad, Patricia A.

In: Parasitology Research, Vol. 87, No. 3, 2001, p. 252-257.

Research output: Contribution to journalArticle

Miller, Melissa A. ; Crosbie, Paul R. ; Sverlow, Karen ; Hanni, Krista ; Barr, Bradd C. ; Kock, Nancy ; Murray, Michael J. ; Lowenstine, Linda J ; Conrad, Patricia A. / Isolation and characterization of Sarcocystis from brain tissue of a free-living southern sea otter (Enhydra lutris nereis) with fatal meningoencephalitis. In: Parasitology Research. 2001 ; Vol. 87, No. 3. pp. 252-257.
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