Abstract
A protozoan was isolated in cell culture from the brain of a free-ranging sea otter with fatal meningoencephalitis. The biological history of this otter, a study animal being monitored via an intraperitoneal radio transmitter, is summarized. Histologically, protozoal parasites were associated with areas of brain inflammation and necrosis in the cerebrum and cerebellum. Morphology and measurements of fixed, Giemsa-stained protozoal zoites growing on coverslips were consistent with Sarcocystis. These parasites reacted only with polyclonal antisera raised against S. neurona on immunohistochemistry. Cell culture-derived zoites reacted strongly with polyclonal antiserum to S. neurona on indirect fluorescent antibody tests. Amplification of portions of the 18S ribosomal DNA and the adjacent first internal transcribed spacer were performed. The resulting sequences were compared with published sequences from similar apicomplexan protozoa. This isolate (SO SN1), was indistinguishable from S. neurona, based on parasite morphology, antigenic reactivity and molecular characterization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 252-257 |
| Number of pages | 6 |
| Journal | Parasitology Research |
| Volume | 87 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2001 |
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- Parasitology
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Isolation and characterization of Sarcocystis from brain tissue of a free-living southern sea otter (Enhydra lutris nereis) with fatal meningoencephalitis. / Miller, Melissa A.; Crosbie, Paul R.; Sverlow, Karen; Hanni, Krista; Barr, Bradd C.; Kock, Nancy; Murray, Michael J.; Lowenstine, Linda J; Conrad, Patricia A.
In: Parasitology Research, Vol. 87, No. 3, 2001, p. 252-257.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of Sarcocystis from brain tissue of a free-living southern sea otter (Enhydra lutris nereis) with fatal meningoencephalitis
AU - Miller, Melissa A.
AU - Crosbie, Paul R.
AU - Sverlow, Karen
AU - Hanni, Krista
AU - Barr, Bradd C.
AU - Kock, Nancy
AU - Murray, Michael J.
AU - Lowenstine, Linda J
AU - Conrad, Patricia A
PY - 2001
Y1 - 2001
N2 - A protozoan was isolated in cell culture from the brain of a free-ranging sea otter with fatal meningoencephalitis. The biological history of this otter, a study animal being monitored via an intraperitoneal radio transmitter, is summarized. Histologically, protozoal parasites were associated with areas of brain inflammation and necrosis in the cerebrum and cerebellum. Morphology and measurements of fixed, Giemsa-stained protozoal zoites growing on coverslips were consistent with Sarcocystis. These parasites reacted only with polyclonal antisera raised against S. neurona on immunohistochemistry. Cell culture-derived zoites reacted strongly with polyclonal antiserum to S. neurona on indirect fluorescent antibody tests. Amplification of portions of the 18S ribosomal DNA and the adjacent first internal transcribed spacer were performed. The resulting sequences were compared with published sequences from similar apicomplexan protozoa. This isolate (SO SN1), was indistinguishable from S. neurona, based on parasite morphology, antigenic reactivity and molecular characterization.
AB - A protozoan was isolated in cell culture from the brain of a free-ranging sea otter with fatal meningoencephalitis. The biological history of this otter, a study animal being monitored via an intraperitoneal radio transmitter, is summarized. Histologically, protozoal parasites were associated with areas of brain inflammation and necrosis in the cerebrum and cerebellum. Morphology and measurements of fixed, Giemsa-stained protozoal zoites growing on coverslips were consistent with Sarcocystis. These parasites reacted only with polyclonal antisera raised against S. neurona on immunohistochemistry. Cell culture-derived zoites reacted strongly with polyclonal antiserum to S. neurona on indirect fluorescent antibody tests. Amplification of portions of the 18S ribosomal DNA and the adjacent first internal transcribed spacer were performed. The resulting sequences were compared with published sequences from similar apicomplexan protozoa. This isolate (SO SN1), was indistinguishable from S. neurona, based on parasite morphology, antigenic reactivity and molecular characterization.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035089379&partnerID=8YFLogxK
U2 - 10.1007/s004360000340
DO - 10.1007/s004360000340
M3 - Article
C2 - 11293576
AN - SCOPUS:0035089379
VL - 87
SP - 252
EP - 257
JO - Zeitschrift fur Parasitenkunde
JF - Zeitschrift fur Parasitenkunde
SN - 0932-0113
IS - 3
ER -