Abstract
Human DNA repair gene XRCC1 complements the strand-break rejoining defect in Chinese hamster mutant EM9 and encodes a protein that is apparently required for optimal activity of DNA ligase III. Toward the goal of producing transgenic mice that carry a mutation in the Xrcc-1 locus, the murine homolog of XRCC1 was cloned from both cosmid genomic and cDNA libraries. Upon transfection into EM9 cells, cosmids containing the functional mouse gene efficiently corrected (94-100%) the high sister-chromatid-exchange defect. Mouse Xrcc-1 is 26 kb in length, contains 17 exons, and maps by metaphase in situ hybridization to the 7A3-7B2 region of mouse chromosome 7. Isolated cDNA clones were highly truncated and were extended by anchored polymerase chain reactions. The 1893-bp open reading frame of mouse Xrcc-1 encodes 631 amino acids, compared with 633 for the human homolog. The predicted mouse Xrcc-1 protein of 69.1 kDa and pI of 5.95 is 86% identical and 93% similar to human XRCC1.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 180-188 |
| Number of pages | 9 |
| Journal | Genomics |
| Volume | 22 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
Isolation and Characterization of Mouse Xrcc-1, a DNA Repair Gene Affecting Ligation. / Brookman, Kerry W.; Tebbs, Robert S.; Allen, Susan A.; Tucker, James D.; Swiger, Roy R.; Lamerdin, Jane E.; Carrano, Anthony V.; Thompson, Larry H.
In: Genomics, Vol. 22, No. 1, 01.07.1994, p. 180-188.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and Characterization of Mouse Xrcc-1, a DNA Repair Gene Affecting Ligation
AU - Brookman, Kerry W.
AU - Tebbs, Robert S.
AU - Allen, Susan A.
AU - Tucker, James D.
AU - Swiger, Roy R.
AU - Lamerdin, Jane E.
AU - Carrano, Anthony V.
AU - Thompson, Larry H.
PY - 1994/7/1
Y1 - 1994/7/1
N2 - Human DNA repair gene XRCC1 complements the strand-break rejoining defect in Chinese hamster mutant EM9 and encodes a protein that is apparently required for optimal activity of DNA ligase III. Toward the goal of producing transgenic mice that carry a mutation in the Xrcc-1 locus, the murine homolog of XRCC1 was cloned from both cosmid genomic and cDNA libraries. Upon transfection into EM9 cells, cosmids containing the functional mouse gene efficiently corrected (94-100%) the high sister-chromatid-exchange defect. Mouse Xrcc-1 is 26 kb in length, contains 17 exons, and maps by metaphase in situ hybridization to the 7A3-7B2 region of mouse chromosome 7. Isolated cDNA clones were highly truncated and were extended by anchored polymerase chain reactions. The 1893-bp open reading frame of mouse Xrcc-1 encodes 631 amino acids, compared with 633 for the human homolog. The predicted mouse Xrcc-1 protein of 69.1 kDa and pI of 5.95 is 86% identical and 93% similar to human XRCC1.
AB - Human DNA repair gene XRCC1 complements the strand-break rejoining defect in Chinese hamster mutant EM9 and encodes a protein that is apparently required for optimal activity of DNA ligase III. Toward the goal of producing transgenic mice that carry a mutation in the Xrcc-1 locus, the murine homolog of XRCC1 was cloned from both cosmid genomic and cDNA libraries. Upon transfection into EM9 cells, cosmids containing the functional mouse gene efficiently corrected (94-100%) the high sister-chromatid-exchange defect. Mouse Xrcc-1 is 26 kb in length, contains 17 exons, and maps by metaphase in situ hybridization to the 7A3-7B2 region of mouse chromosome 7. Isolated cDNA clones were highly truncated and were extended by anchored polymerase chain reactions. The 1893-bp open reading frame of mouse Xrcc-1 encodes 631 amino acids, compared with 633 for the human homolog. The predicted mouse Xrcc-1 protein of 69.1 kDa and pI of 5.95 is 86% identical and 93% similar to human XRCC1.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027965161&partnerID=8YFLogxK
U2 - 10.1006/geno.1994.1359
DO - 10.1006/geno.1994.1359
M3 - Article
C2 - 7959765
AN - SCOPUS:0027965161
VL - 22
SP - 180
EP - 188
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -