Isolation and characterization of granuloma initiation factor

Maxwell A Fung, Naoyoshi Sato, Toshihiro Iida, Kimie Fukuyama, William L. Epstein

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A soluble component that transfers granulomatous tissue reaction was fractionated from Schistosoma mansoni egg-induced hepatic granulomas (SMHG) by Sephacryl S-300 column chromatography. The fractions separately bound to inert, Affi-Gel agarose beads were inoculated subcutaneously in naive mice. The low molecular weight fraction, consisting of proteins 23 kd, 20 kd, and 16 kd, produced organized granulomas 6 to 7 weeks after inoculation. This fraction was further purified by high-pressure liquid chromatograpby (HPLC) gel filtration and gave three fractions eluting at retention times of 44, 46, and 48 minutes. Each fraction contained all low-molecularweight proteins in varying amounts and induced skin granulomas when inoculated subcutaneously. Amino acid sequence of the major 20-kd protein showed 11 N-terminal residues identical to those of cyclophilin. Antisera raised to the protein with retention time of 46 minutes, reacted with cells in the granulomas but not surrounding liver tissue as detected by immunofluorescence microscopy. The findings indicate a low molecular weight soluble fraction of SMHG can induce new granuloma formation when injected in an immobilized form into skin of naive mice. The results suggest granuloma initiation factor is a homolog of the cyclophilin gene family.

Original languageEnglish (US)
Pages (from-to)1445-1451
Number of pages7
JournalAmerican Journal of Pathology
Volume141
Issue number6
StatePublished - Dec 1992
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Fung, M. A., Sato, N., Iida, T., Fukuyama, K., & Epstein, W. L. (1992). Isolation and characterization of granuloma initiation factor. American Journal of Pathology, 141(6), 1445-1451.