Isolation and characterization of cat milk proteins

Y. Adkins, B. Lönnerdal, A. J. Lepine

Research output: Contribution to journalArticle

Abstract

Miik proteins have important nutritional and physiological roles in the newborn, ranging from providing amino acids for facilitating nutrient uptake and development of the gut. Our knowledge regarding the proteins in cat milk is still very limited. In this study, mature cat milk samples (1 mo of lactation) were collected from domestic shorthair cats. A method was developed for efficient separation of casein and whey proteins by adjustment of the pH to 4.6 followed by ultracentrifugation. Fast Protein Liquid Chromatography (FPLC) using an anion-exchange column (Mono-Q) was used to separate the proteins. Purity of isolated proteins was assessed by polyacrylamide gradient gel electrophoresis. Amino acid composition was analyzed following acid hydrolysis using a Beckman 3600 Analyzer. The major whey proteins with MWs of 14, 19, 20, and 78 kD were isolated to purity. Comparison of their amino acid compositions to known whey proteins from other species suggest that these are lysozyme, β-lactoglobulin A and B, and transferrin.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

Milk Proteins
milk proteins
Cats
cats
whey protein
Proteins
amino acid composition
Amino Acids
proteins
purity
Milk
lactoglobulins
milk
Lactoglobulins
ultracentrifugation
Ultracentrifugation
transferrin
acid hydrolysis
anion exchange
polyacrylamide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Adkins, Y., Lönnerdal, B., & Lepine, A. J. (1996). Isolation and characterization of cat milk proteins. FASEB Journal, 10(3).

Isolation and characterization of cat milk proteins. / Adkins, Y.; Lönnerdal, B.; Lepine, A. J.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Adkins, Y, Lönnerdal, B & Lepine, AJ 1996, 'Isolation and characterization of cat milk proteins', FASEB Journal, vol. 10, no. 3.
Adkins Y, Lönnerdal B, Lepine AJ. Isolation and characterization of cat milk proteins. FASEB Journal. 1996;10(3).
Adkins, Y. ; Lönnerdal, B. ; Lepine, A. J. / Isolation and characterization of cat milk proteins. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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