Isolation and characterization of bovine choroidal microvessel endothelium and pericytes in culture

Lawrence S Morse, Yossi Sidikaro

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The invasion of Bruch's membrane and subsequently the subpigment epithelial and subretinal spaces by proliferating choroidal capillaries are prominent features of age-related macular degeneration. To facilitate study of this process, pure cell lines of bovine choroidal microvessel endothelium and pericytes were isolated and propagated in cell culture media. Their biological and biochemical properties are described. Choroidal microvascular endothelial cells displayed the characteristic cobblestone morphology, required fibronectin or gelatin for attachment to plastic culture dishes, displayed contact inhibition of growth and spontaneously formed tubules in culture. These cells contained antigens for Factor VIII and were able to phagocytize acetylated-low density lipoprotein. Choroidal microvascular pericytes were nonrectangular in shape and did not require substrate attachment factors. They were unable to form tubules in culture, were Factor VIII antigen negative, were unable to phagocytize acetylated-low density lipoprotein and did not display contact inhibition of growth. Choroidal microvascular pericytes contained antigens for muscle and nonmuscle actin.

Original languageEnglish (US)
Pages (from-to)631-642
Number of pages12
JournalCurrent Eye Research
Volume9
Issue number7
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Pericytes
Microvessels
Contact Inhibition
Endothelium
Factor VIII
Antigens
Bruch Membrane
Macular Degeneration
Gelatin
Growth
Fibronectins
Plastics
Culture Media
Actins
Endothelial Cells
Cell Culture Techniques
Cell Line
Muscles
acetyl-LDL

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Isolation and characterization of bovine choroidal microvessel endothelium and pericytes in culture. / Morse, Lawrence S; Sidikaro, Yossi.

In: Current Eye Research, Vol. 9, No. 7, 1990, p. 631-642.

Research output: Contribution to journalArticle

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