Abstract
We have isolated the first mosquito member of the TGF-β superfamily, As60A. As60A is a single copy gene, ∼5 kb in length and encodes eight exons. Here we report the isolation and characterization of two of four transcripts produced from this gene. The transcripts As60A1 and As60A2 encode related 5'UTR/exon 1 sequences. As60A is most similar to the 60A genes from Drosophila and is thus a member of the Dpp/BMP subfamily of the TGF-β superfamily. The splice junction of intron 2 is conserved among As60A, BMP2, BMP4, Tc-Dpp, Bm-tgh-1, TGF-β1 and Dpp. Intron 2 also contains three putative binding sites for a Dorsal/Gambif1 transcription factor. The large number of introns and the conservation of intron 2 indicate that As60A is relatively ancient compared to the other arthropod TGF-β genes. We also propose that As60A plays a role in the mosquito immune response to Plasmodium infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 65-74 |
| Number of pages | 10 |
| Journal | Cytokine |
| Volume | 13 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 21 2001 |
| Externally published | Yes |
Fingerprint
Keywords
- 60A
- Arthropod
- Malaria
- Mosquito
- TGF-β
ASJC Scopus subject areas
- Endocrinology
- Molecular Biology
- Immunology
- Immunology and Allergy
Cite this
Isolation and characterization of As60A, a transforming growth factor-β gene, from the malaria vector Anopheles stephensi. / Crampton, A. L.; Luckhart, S.
In: Cytokine, Vol. 13, No. 2, 21.01.2001, p. 65-74.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of As60A, a transforming growth factor-β gene, from the malaria vector Anopheles stephensi
AU - Crampton, A. L.
AU - Luckhart, S.
PY - 2001/1/21
Y1 - 2001/1/21
N2 - We have isolated the first mosquito member of the TGF-β superfamily, As60A. As60A is a single copy gene, ∼5 kb in length and encodes eight exons. Here we report the isolation and characterization of two of four transcripts produced from this gene. The transcripts As60A1 and As60A2 encode related 5'UTR/exon 1 sequences. As60A is most similar to the 60A genes from Drosophila and is thus a member of the Dpp/BMP subfamily of the TGF-β superfamily. The splice junction of intron 2 is conserved among As60A, BMP2, BMP4, Tc-Dpp, Bm-tgh-1, TGF-β1 and Dpp. Intron 2 also contains three putative binding sites for a Dorsal/Gambif1 transcription factor. The large number of introns and the conservation of intron 2 indicate that As60A is relatively ancient compared to the other arthropod TGF-β genes. We also propose that As60A plays a role in the mosquito immune response to Plasmodium infection.
AB - We have isolated the first mosquito member of the TGF-β superfamily, As60A. As60A is a single copy gene, ∼5 kb in length and encodes eight exons. Here we report the isolation and characterization of two of four transcripts produced from this gene. The transcripts As60A1 and As60A2 encode related 5'UTR/exon 1 sequences. As60A is most similar to the 60A genes from Drosophila and is thus a member of the Dpp/BMP subfamily of the TGF-β superfamily. The splice junction of intron 2 is conserved among As60A, BMP2, BMP4, Tc-Dpp, Bm-tgh-1, TGF-β1 and Dpp. Intron 2 also contains three putative binding sites for a Dorsal/Gambif1 transcription factor. The large number of introns and the conservation of intron 2 indicate that As60A is relatively ancient compared to the other arthropod TGF-β genes. We also propose that As60A plays a role in the mosquito immune response to Plasmodium infection.
KW - 60A
KW - Arthropod
KW - Malaria
KW - Mosquito
KW - TGF-β
UR - http://www.scopus.com/inward/record.url?scp=0035924919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035924919&partnerID=8YFLogxK
U2 - 10.1006/cyto.2000.0801
DO - 10.1006/cyto.2000.0801
M3 - Article
C2 - 11145845
AN - SCOPUS:0035924919
VL - 13
SP - 65
EP - 74
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 2
ER -