Isolation and characterization of As60A, a transforming growth factor-β gene, from the malaria vector Anopheles stephensi

A. L. Crampton, S. Luckhart

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have isolated the first mosquito member of the TGF-β superfamily, As60A. As60A is a single copy gene, ∼5 kb in length and encodes eight exons. Here we report the isolation and characterization of two of four transcripts produced from this gene. The transcripts As60A1 and As60A2 encode related 5'UTR/exon 1 sequences. As60A is most similar to the 60A genes from Drosophila and is thus a member of the Dpp/BMP subfamily of the TGF-β superfamily. The splice junction of intron 2 is conserved among As60A, BMP2, BMP4, Tc-Dpp, Bm-tgh-1, TGF-β1 and Dpp. Intron 2 also contains three putative binding sites for a Dorsal/Gambif1 transcription factor. The large number of introns and the conservation of intron 2 indicate that As60A is relatively ancient compared to the other arthropod TGF-β genes. We also propose that As60A plays a role in the mosquito immune response to Plasmodium infection.

Original languageEnglish (US)
Pages (from-to)65-74
Number of pages10
JournalCytokine
Volume13
Issue number2
DOIs
StatePublished - Jan 21 2001
Externally publishedYes

Fingerprint

Anopheles
Transforming Growth Factors
Introns
Malaria
Genes
Culicidae
Exons
Arthropods
5' Untranslated Regions
Drosophila
Conservation
Transcription Factors
Binding Sites

Keywords

  • 60A
  • Arthropod
  • Malaria
  • Mosquito
  • TGF-β

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Isolation and characterization of As60A, a transforming growth factor-β gene, from the malaria vector Anopheles stephensi. / Crampton, A. L.; Luckhart, S.

In: Cytokine, Vol. 13, No. 2, 21.01.2001, p. 65-74.

Research output: Contribution to journalArticle

@article{2ead395b5a154ff0b7fb4658da2f6421,
title = "Isolation and characterization of As60A, a transforming growth factor-β gene, from the malaria vector Anopheles stephensi",
abstract = "We have isolated the first mosquito member of the TGF-β superfamily, As60A. As60A is a single copy gene, ∼5 kb in length and encodes eight exons. Here we report the isolation and characterization of two of four transcripts produced from this gene. The transcripts As60A1 and As60A2 encode related 5'UTR/exon 1 sequences. As60A is most similar to the 60A genes from Drosophila and is thus a member of the Dpp/BMP subfamily of the TGF-β superfamily. The splice junction of intron 2 is conserved among As60A, BMP2, BMP4, Tc-Dpp, Bm-tgh-1, TGF-β1 and Dpp. Intron 2 also contains three putative binding sites for a Dorsal/Gambif1 transcription factor. The large number of introns and the conservation of intron 2 indicate that As60A is relatively ancient compared to the other arthropod TGF-β genes. We also propose that As60A plays a role in the mosquito immune response to Plasmodium infection.",
keywords = "60A, Arthropod, Malaria, Mosquito, TGF-β",
author = "Crampton, {A. L.} and S. Luckhart",
year = "2001",
month = "1",
day = "21",
doi = "10.1006/cyto.2000.0801",
language = "English (US)",
volume = "13",
pages = "65--74",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Isolation and characterization of As60A, a transforming growth factor-β gene, from the malaria vector Anopheles stephensi

AU - Crampton, A. L.

AU - Luckhart, S.

PY - 2001/1/21

Y1 - 2001/1/21

N2 - We have isolated the first mosquito member of the TGF-β superfamily, As60A. As60A is a single copy gene, ∼5 kb in length and encodes eight exons. Here we report the isolation and characterization of two of four transcripts produced from this gene. The transcripts As60A1 and As60A2 encode related 5'UTR/exon 1 sequences. As60A is most similar to the 60A genes from Drosophila and is thus a member of the Dpp/BMP subfamily of the TGF-β superfamily. The splice junction of intron 2 is conserved among As60A, BMP2, BMP4, Tc-Dpp, Bm-tgh-1, TGF-β1 and Dpp. Intron 2 also contains three putative binding sites for a Dorsal/Gambif1 transcription factor. The large number of introns and the conservation of intron 2 indicate that As60A is relatively ancient compared to the other arthropod TGF-β genes. We also propose that As60A plays a role in the mosquito immune response to Plasmodium infection.

AB - We have isolated the first mosquito member of the TGF-β superfamily, As60A. As60A is a single copy gene, ∼5 kb in length and encodes eight exons. Here we report the isolation and characterization of two of four transcripts produced from this gene. The transcripts As60A1 and As60A2 encode related 5'UTR/exon 1 sequences. As60A is most similar to the 60A genes from Drosophila and is thus a member of the Dpp/BMP subfamily of the TGF-β superfamily. The splice junction of intron 2 is conserved among As60A, BMP2, BMP4, Tc-Dpp, Bm-tgh-1, TGF-β1 and Dpp. Intron 2 also contains three putative binding sites for a Dorsal/Gambif1 transcription factor. The large number of introns and the conservation of intron 2 indicate that As60A is relatively ancient compared to the other arthropod TGF-β genes. We also propose that As60A plays a role in the mosquito immune response to Plasmodium infection.

KW - 60A

KW - Arthropod

KW - Malaria

KW - Mosquito

KW - TGF-β

UR - http://www.scopus.com/inward/record.url?scp=0035924919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035924919&partnerID=8YFLogxK

U2 - 10.1006/cyto.2000.0801

DO - 10.1006/cyto.2000.0801

M3 - Article

C2 - 11145845

AN - SCOPUS:0035924919

VL - 13

SP - 65

EP - 74

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 2

ER -