Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus

Katherine M. Call, Thomas M Glaser, Caryn Y. Ito, Alan J. Buckler, Jerry Pelletier, Daniel A. Haber, Elise A. Rose, Astrid Kral, Herman Yeger, William H. Lewis, Carol Jones, David E. Housman

Research output: Contribution to journalArticlepeer-review

1574 Scopus citations

Abstract

We have isolated a series of genomic and cDNA clones mapping within the boundaries of constitutional and tumor deletions that define the Wilms' tumor locus on human chromsome 11 (band p13). The transcription unit corresponding to these clones spans approximately 50 kb and encodes an mRNA approximately 3 kb long. This mRNA is expressed in a limited range of cell types, predominantly in the kidney and a subset of hematopoietic cells. The polypeptide encoded by this locus has a number of features suggesting a potential role in transcriptional regulation. These include the presence of four zinc finger domains and a region rich in proline and glutamine. The amino acid sequence of the predicted polypeptide shows significant homology to two growth regulated mammalian polypeptides, EGR1 and EGR2. The genetic localization of this gene, its tissue-specific expression, and the function predicted from its sequence lead us to suggest that it represents the 11p13 Wilms' tumor gene.

Original languageEnglish (US)
Pages (from-to)509-520
Number of pages12
JournalCell
Volume60
Issue number3
DOIs
StatePublished - Feb 9 1990
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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