Isolation and characterization of a protease from Pseudomonas fluorescens RO98

R. Koka, Bart C Weimer

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Pseudomonas fluorescens RO98, a raw milk isolate, was inoculated into McKellar's minimal salts medium and incubated at 25°C for 48 h to allow production of protease. A zinc-metalloacid protease was purified from the cell-free concentrate by anion exchange and gel filtration chromatography. The purified protease was active between 15 and 55°C, and pH4.5 and 9.0, and was stable to pasteurization. The enzyme had pH and temperature optima for activity of 5·0 and 35°C, respectively. It was heat stable with a D55 of 41 min and a D(62·5) of 18 h. Molecular weight of the enzyme was estimated to be 52 kDa by SDS PAGE and size exclusion chromatography. Values for k(M) of 144·28, 18·73, 110·20 and 35·23 μmol were obtained for whole, α-, β- and κ-casein, with a V(max) of 8·26, 0·09, 0·42 and 0·70 μmol mg-1 min-1, respectively. The enzyme hydrolysed κ-casein preferentially when incubated with artificial casein micelles.

Original languageEnglish (US)
Pages (from-to)280-288
Number of pages9
JournalJournal of Applied Microbiology
Volume89
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Pseudomonas fluorescens
Caseins
casein
Peptide Hydrolases
proteinases
Gel Chromatography
Enzymes
enzymes
Pasteurization
Micelles
micelles
anion exchange
pasteurization
raw milk
Anions
Zinc
Polyacrylamide Gel Electrophoresis
Milk
Salts
concentrates

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Isolation and characterization of a protease from Pseudomonas fluorescens RO98. / Koka, R.; Weimer, Bart C.

In: Journal of Applied Microbiology, Vol. 89, No. 2, 2000, p. 280-288.

Research output: Contribution to journalArticle

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