Abstract
We have isolated a cloned segment of the chick α1 type III collagen gene from a genomic DNA library. This DNA clone was obtained by cross-hybridization with a cDNA clone containing sequences coding for a segment of the carboxy propeptide of chick α1 type I collagen. This genomic clone was identified as coding for α1 type III collagen by DNA sequence analysis of two segments of the gene and by its ability to hybridize to crop mRNA but not to calvaria mRNA in agreement with the presence of α1 type III collagen in crop but not in calvaria. The deduced amino acid sequences reveal characteristic features for α1 type III collagen and are in good agreement with the known protein sequences of calf and human α1 type III collagen. The size of α1 type III mRNA is about 6 kilobases, slightly larger than the mRNA for α1 type I. R-loop electron microscopic analysis indicates that at least 16 exons are interspersed in a 9-kilobase DNA segment that contains the third distal part of the α1 type III collagen gene.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2758-2761 |
| Number of pages | 4 |
| Journal | Journal of Biological Chemistry |
| Volume | 258 |
| Issue number | 5 |
| State | Published - 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Isolation and characterization of a genomic clone encoding chick α1 type III collagen. / Yamada, Y.; Mudryj, Maria; Sullivan, M.; De Crombrugghe, B.
In: Journal of Biological Chemistry, Vol. 258, No. 5, 1983, p. 2758-2761.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of a genomic clone encoding chick α1 type III collagen
AU - Yamada, Y.
AU - Mudryj, Maria
AU - Sullivan, M.
AU - De Crombrugghe, B.
PY - 1983
Y1 - 1983
N2 - We have isolated a cloned segment of the chick α1 type III collagen gene from a genomic DNA library. This DNA clone was obtained by cross-hybridization with a cDNA clone containing sequences coding for a segment of the carboxy propeptide of chick α1 type I collagen. This genomic clone was identified as coding for α1 type III collagen by DNA sequence analysis of two segments of the gene and by its ability to hybridize to crop mRNA but not to calvaria mRNA in agreement with the presence of α1 type III collagen in crop but not in calvaria. The deduced amino acid sequences reveal characteristic features for α1 type III collagen and are in good agreement with the known protein sequences of calf and human α1 type III collagen. The size of α1 type III mRNA is about 6 kilobases, slightly larger than the mRNA for α1 type I. R-loop electron microscopic analysis indicates that at least 16 exons are interspersed in a 9-kilobase DNA segment that contains the third distal part of the α1 type III collagen gene.
AB - We have isolated a cloned segment of the chick α1 type III collagen gene from a genomic DNA library. This DNA clone was obtained by cross-hybridization with a cDNA clone containing sequences coding for a segment of the carboxy propeptide of chick α1 type I collagen. This genomic clone was identified as coding for α1 type III collagen by DNA sequence analysis of two segments of the gene and by its ability to hybridize to crop mRNA but not to calvaria mRNA in agreement with the presence of α1 type III collagen in crop but not in calvaria. The deduced amino acid sequences reveal characteristic features for α1 type III collagen and are in good agreement with the known protein sequences of calf and human α1 type III collagen. The size of α1 type III mRNA is about 6 kilobases, slightly larger than the mRNA for α1 type I. R-loop electron microscopic analysis indicates that at least 16 exons are interspersed in a 9-kilobase DNA segment that contains the third distal part of the α1 type III collagen gene.
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M3 - Article
VL - 258
SP - 2758
EP - 2761
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 5
ER -