TY - JOUR
T1 - Isolation and characterization of α-fetoprotein from the mouse hepatoma bw7756
AU - Allen, Roblee P
AU - Mizejewski, G. J.
PY - 1977/3/28
Y1 - 1977/3/28
N2 - Purification of α-fetoprotein from mouse hepatoma BW7756 extracts was performed using ammonium sulfate precipitation, gel filtration, ion-exchange chromatography and isoelectric focusing. These procedures produced a 5.6% yield of α-fetoprotein with 96% purity. Polyacrylamide slab gel electrophoresis, extended agarose electrophoresis and immunoelectrophoresis demonstrated that mouse hepatoma α-fetoprotein migrated at pH 8.6 as a rapid α1, or postalbumin globulin. Crossed antibody electrophoresis of the agarose zone containing α-fetoprotein failed to demonstrate microheterogeneity. Molecular weight analysis of the mouse hepatoma α-fetoprotein on a calibrated Sephadex G-200 column yielded a value of 72 000-73 000 for the native protein. Sodium dodecyl sulfate gel electrophoresis subsequently demonstrated a single polypeptide chain with a molecular weight of 72 000. Amino acid analysis showed the α-fetoprotein to be an acidic protein dominated by hydrophobic residues. The total carbohydrate content was 5.5% and 3 mol of sialic acid were detected per mol of α-fetoprotein. Although neutral sugars were the principal class present, galactosamine was the most abundant single sugar detected.
AB - Purification of α-fetoprotein from mouse hepatoma BW7756 extracts was performed using ammonium sulfate precipitation, gel filtration, ion-exchange chromatography and isoelectric focusing. These procedures produced a 5.6% yield of α-fetoprotein with 96% purity. Polyacrylamide slab gel electrophoresis, extended agarose electrophoresis and immunoelectrophoresis demonstrated that mouse hepatoma α-fetoprotein migrated at pH 8.6 as a rapid α1, or postalbumin globulin. Crossed antibody electrophoresis of the agarose zone containing α-fetoprotein failed to demonstrate microheterogeneity. Molecular weight analysis of the mouse hepatoma α-fetoprotein on a calibrated Sephadex G-200 column yielded a value of 72 000-73 000 for the native protein. Sodium dodecyl sulfate gel electrophoresis subsequently demonstrated a single polypeptide chain with a molecular weight of 72 000. Amino acid analysis showed the α-fetoprotein to be an acidic protein dominated by hydrophobic residues. The total carbohydrate content was 5.5% and 3 mol of sialic acid were detected per mol of α-fetoprotein. Although neutral sugars were the principal class present, galactosamine was the most abundant single sugar detected.
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U2 - 10.1016/0005-2795(77)90060-5
DO - 10.1016/0005-2795(77)90060-5
M3 - Article
C2 - 66068
AN - SCOPUS:0017338684
VL - 491
SP - 242
EP - 252
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
SN - 1570-9639
IS - 1
ER -